cDNA Clone RNF146

• Plasmid: pEFIRES-P-eYFP C1-RNF146
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-RNF146
• Genbank: NM_030963.2
• Tag: YFP
• Species: Human

Sequence of Insert

GCGGCCGCATGGCTGGCTGTGGTGAAATTGATCATTCAATAAACATGCTT
CCTACAAACAGGAAAGCGAACGAGTCCTGTTCTAATACTGCACCTTCTTT
AACCGTCCCTGAATGTGCCATTTGTCTGCAAACATGTGTTCATCCAGTCA
GTCTGCCCTGTAAGCACGTTTTCTGCTATCTATGTGTAAAAGGAGCTTCA
TGGCTTGGAAAGCGGTGTGCTCTTTGTCGACAAGAAATTCCCGAGGATTT
CCTTGACAAGCCAACCTTGTTGTCACCAGAAGAACTCAAGGCAGCAAGTA
GAGGAAATGGTGAATATGCATGGTATTATGAAGGAAGAAATGGGTGGTGG
CAGTACGATGAGCGCACTAGTAGAGAGCTGGAAGATGCTTTTTCCAAAGG
TAAAAAGAACACTGAAATGTTAATTGCTGGCTTTCTGTATGTCGCTGATC
TTGAAAACATGGTTCAATATAGGAGAAATGAACATGGACGTCGCAGGAAG
ATTAAGCGAGATATAATAGATATACCAAAGAAGGGAGTAGCTGGACTTAG
GCTAGACTGTGATGCTAATACCGTAAACCTAGCAAGAGAGAGCTCTGCTG
ACGGAGCGGACAGTGTATCAGCACAGAGTGGAGCTTCTGTTCAGCCCCTA
GTGTCTTCTGTAAGGCCCCTAACATCAGTAGATGGTCAGTTAACAAGCCC
TGCAACACCATCCCCTGATGCAAGCACTTCTCTGGAAGACTCTTTTGCTC
ATTTACAACTCAGTGGAGACAACACAGCTGAAAGGAGTCATAGGGGAGAA
GGAGAAGAAGATCATGAATCACCATCTTCAGGCAGGGTACCAGCACCAGA
CACCTCCATTGAAGAAACTGAATCAGATGCCAGTAGTGATAGTGAGGATG
TATCTGCAGTTGTTGCACAGCACTCCTTGACCCAACAGAGACTTTTGGTT
TCTAATGCAAACCAGACAGTACCCGATCGATCAGATCGATCGGGAACTGA
TCGATCAGTAGCAGGGGGTGGAACAGTGAGTGTCAGTGTCAGATCTAGAA
GGCCTGATGGACAGTGCACAGTAACTGAAGTTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSTGSRVDPGGRMAGCGEIDHSINMLPTNRKANESCSNTAP
SLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPE
DFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELEDAFS
KGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAG
LRLDCDANTVNLARESSADGADSVSAQSGASVQPLVSSVRPLTSVDGQLT
SPATPSPDASTSLEDSFAHLQLSGDNTAERSHRGEGEEDHESPSSGRVPA
PDTSIEETESDASSDSEDVSAVVAQHSLTQQRLLVSNANQTVPDRSDRSG
TDRSVAGGGTVSVSVRSRRPDGQCTVTEV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20596
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP-RNF146. EF1a promoter, IRES and puromycin selection cassette; ; Estimated MW of expressed protein : 68804 Daltons