cDNA Clone optineurin

• Plasmid: pcDNA5-FRT/TO-DAC-A-C3 Optineurin
• Parent Plasmid: pcDNA5 FRT/TO DAC 3C
• Expressed: DAC-A-C3 Optineurin
• Genbank: AF420371
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGTCCCATCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAG
CCCCAGTGAAAGCACAGGAAATGGACCCCCCCACCTGGCCCACCCAAACC
TGGACACGTTTACCCCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTG
ACCGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCAT
GAAAGGGAGATTTGAGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAG
AACGCCAGTTTTTTGAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATG
GCCTTGAGTCATGAGAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAA
AGGGAAATCAGAAAGGTCATCTGAGGACCCCACTGATGACTCCAGGCTTC
CCAGGGCCGAAGCGGAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTG
AGGCTACAAGCAGAGAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCA
GCTCAAGCTGAACTCCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTA
GGATGGCTGAAGGAGAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGT
CCTGGGCCCACGAGAACAGTCTCCACTGGCACGGCATTGTCTAAATATAG
GAGCAGATCTGCAGATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAA
CTGTGAGCCAGCTCCTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAG
AGACTTGAAGTTGCACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGA
AAAGAAAACAAGTAATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCA
CAGAGAAAGAGAATGATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAA
GTGGAAGCACTGAACCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGA
GGCTCATACAAAACTCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAG
AAAAGTGTCAGGCCCTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTG
AATGAAAAGCAAGAGCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGT
GGAAAGCATGCTATCAGAAATCAAAATGGAACAGGCTAAAACAGAGGATG
AAAAGTCCAAATTAACTGTGCTACAGATGACACACAACAAGCTTCTTCAA
GAACATAATAATGCATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTC
AGAAAAAGTGGACAGGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAAC
TGGCAGAGAAGGCTCTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAG
CAAACCATTGCCAAGCAGGAAGAGGACCTGGAAACCATGACCATCCTCAG
GGCTCAGATGGAAGTTTACTGTTCTGATTTTCATGCTGAAAGAGCAGCGA
GAGAGAAAATTCATGAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTT
CTGCTGAAAGAGAATGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGAT
GGAGATGCAGAGTCGTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGG
CTTACCTTGTTCAAAGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGG
AATATTCCGATTCATTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACAT
AGACACGTTACAGATTCACGTGATGGATTGCATCATTTAAGCGGCCGC

Amino Acid Sequence

MIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQA
MKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGIN
LQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADG
QYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSL
NVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLT
WGFRFFETVNPLEVLFQGPGSMSHQPLSCLTEKEDSPSESTGNGPPHLAH
PNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQ
KEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDS
RLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFV
EIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHE
ELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTE
GSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKR
LQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKT
EDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEK
LELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAER
AAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSD
QQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20573
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Flp-In vector for mammalian expression of N-terminally DAC-A tagged optineurin; ; Estimated MW of expressed protein : 95410 Daltons; Parental Plasmid DU20573