cDNA Clone Ubiquitin

• Plasmid: pcDNA5-FRT/TO-DAC-A-C3 Ubiquitin G75A G76A octomer
• Parent Plasmid: pcDNA5 FRT/TO DAC 3C
• Expressed: DAC-A-C3 Ubiquitin G75A G76A octomer
• Genbank: P62988
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCGGCGCC ATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGT
GGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGG
AAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCC
TGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAG
AATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCA
GAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTG
ACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGA
GCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGA
AGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACC
ATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGA
CCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCC
TGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGT
CTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGCCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAA
GACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGG
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC
TTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACAT
CCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCCGCCATGC
AGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAG
CCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGG
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG
ATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCAC
CTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGGCCGC

Amino Acid Sequence

MIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQA
MKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGIN
LQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADG
QYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSL
NVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLT
WGFRFFETVNPLEVLFQGPGSGAMQIFVKTLTGKTITLEVEPSDTIENVK
AKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRAAM
QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLE
DGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTIEN
VKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRA
AMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQ
LEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRAAMQIFVKTLTGKTITLEVEPSD
TIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVL
RLRAAMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLSDYNIQKESTLHLVLRLRAA**

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20572
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Flp-In vector for mammalian expression of N-terminally DAC-A tagged G75A G76A octoubiquitin (with initiating methionines); ; Estimated MW of expressed protein : 98234 Daltons; Parental Plasmid DU20572