cDNA Clone CDC4

• Plasmid: pYES2-StrepII-6His.TUBE-CDC4
• Parent Plasmid: pYES2
• Expressed: StrepII.6His.TUBE-CDC4
• Genbank: 850539 (NP_116585)
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGGGGTCGTTTCCCTTAGCTGAGTTTCCATTACGTGATATCCC
TGTTCCTTATAGCTACCGTGTGTCTGGCGGTATAGCTTCCTCAGGTAGTG
TTACTGCGCTTGTTACTGCCGCTGGCACTCATCGAAACTCGTCCACGGCT
AAGACAGTTGAGACAGAAGACGGCGAAGAAGATATCGATGAGTATCAGAG
GAAAAGAGCAGCTGGTTCTGGCGAATCCACTCCTGAACGCAGTGATTTCA
AAAGGGTAAAACATGATAATCACAAAACCCTCCATCCAGTTAACTTACAG
AACACCGGTGCAGCGTCTGTGGATAATGACGGTCTGCACAATTTAACAGA
TATATCCAACGATGCAGAAAAACTTTTGATGTCTGTGGATGATGGTTCTG
CCGCACCTTCTACATTGAGTGTAAACATGGGAGTGGCATCTCATAATGTT
GCTGCTCCCACTACCGTCAATGCGGCAACAATAACTGGCAGTGATGTTAG
TAACAATGTTAATAGTGCTACTATTAACAATCCTATGGAGGAAGGAGCGC
TGCCGTTATCACCCACTGCTTCCTCTCCAGGTACCACAACTCCTTTAGCT
AAAACTACGAAAACTATCAACAACAATAATAATATCGCCGATTTGATAGA
ATCCAAAGATTCTATAATCTCCCCTGAATACCTTTCTGATGAGATTTTCA
GCGCAATAAACAATAATCTCCCTCACGCATACTTCAAAAATTTATTATTT
AGATTAGTTGCCAACATGGATAGGAGTGAACTATCCGACTTGGGGACTTT
AATCAAGGATAATTTAAAGAGGGACCTAATAACGTCTTTGCCTTTTGAAA
TAAGTTTGAAAATTTTCAATTATTTGCAATTCGAGGATATTATAAATTCC
CTTGGGGTCTCCCAAAATTGGAACAAAATAATTAGAAAATCTACATCGTT
GTGGAAAAAACTTCTGATATCGGAAAATTTTGTGAGCCCAAAGGGTTTTA
ATTCTCTCAATCTCAAACTCTCCCAAAAATACCCAAAACTCTCACAACAA
GATCGCCTTAGATTATCTTTTCTGGAGAATATATTCATTTTAAAAAATTG
GTACAATCCCAAGTTTGTACCACAAAGGACCACGTTAAGAGGCCATATGA
CGAGTGTTATTACGTGCTTGCAATTTGAAGATAATTATGTCATTACGGGG
GCTGATGACAAAATGATCAGAGTTTATGATTCGATAAACAAGAAATTTCT
TCTACAACTATCAGGTCATGATGGTGGGGTTTGGGCATTGAAGTATGCCC
ATGGCGGTATTTTAGTCAGCGGTTCTACAGACAGAACGGTGCGAGTTTGG
GATATTAAGAAAGGTTGTTGTACCCATGTGTTTAAAGGTCATAACTCTAC
GGTGAGGTGCCTAGATATAGTAGAATATAAAAATATCAAGTACATTGTTA
CTGGTTCGAGAGATAACACTTTGCACGTTTGGAAATTGCCCAAGGAGTCC
TCCGTTCCTGATCATGGGGAAGAACATGATTATCCATTAGTCTTTCATAC
CCCTGAGGAGAACCCATATTTTGTTGGTGTTTTAAGAGGACATATGGCAT
CTGTAAGAACTGTCTCAGGCCACGGTAATATTGTCGTTAGTGGCTCCTAT
GATAATACACTGATTGTGTGGGATGTTGCGCAAATGAAATGTTTGTATAT
TTTAAGTGGACATACGGATCGTATTTATTCGACAATCTACGATCATGAAA
GAAAAAGGTGCATCTCTGCCAGTATGGATACCACTATTAGAATTTGGGAT
TTGGAAAATATATGGAATAATGGAGAATGTTCCTACGCAACAAATTCAGC
ATCGCCATGCGCCAAAATACTTGGTGCTATGTACACTTTGCAGGGTCATA
CAGCTTTGGTCGGTTTATTAAGATTATCCGACAAATTTTTGGTCAGTGCC
GCTGCAGACGGTTCAATAAGGGGTTGGGACGCAAACGACTACTCTAGAAA
ATTTTCCTACCATCATACCAATTTGAGTGCAATTACCACATTTTATGTAT
CGGATAATATTTTGGTGAGTGGATCGGAAAATCAGTTCAACATCTATAAT
CTACGGAGTGGGAAATTGGTCCACGCAAATATTCTAAAAGATGCTGATCA
GATTTGGTCGGTTAATTTTAAGGGCAAAACACTTGTTGCAGCAGTTGAAA
AAGATGGACAGAGCTTTTTAGAAATTCTGGATTTCAGCAAAGCTTCAAAA
ATTAACTACGTTAGCAATCCCGTAAACTCCTCGTCGTCGTCTTTGGAATC
CATTTCTACTTCTTTGGGTCTAACGAGGACAACTATAATACCATGAGCGG
CCGC

Amino Acid Sequence

MWSHPQFEKGAHHHHHHRSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNRE
ANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQL
EQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQ
LQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS
GGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDIN
AAIERLLGSQPSGGGGSGGGGGGGGSMGSFPLAEFPLRDIPVPYSYRVSG
GIASSGSVTALVTAAGTHRNSSTAKTVETEDGEEDIDEYQRKRAAGSGES
TPERSDFKRVKHDNHKTLHPVNLQNTGAASVDNDGLHNLTDISNDAEKLL
MSVDDGSAAPSTLSVNMGVASHNVAAPTTVNAATITGSDVSNNVNSATIN
NPMEEGALPLSPTASSPGTTTPLAKTTKTINNNNNIADLIESKDSIISPE
YLSDEIFSAINNNLPHAYFKNLLFRLVANMDRSELSDLGTLIKDNLKRDL
ITSLPFEISLKIFNYLQFEDIINSLGVSQNWNKIIRKSTSLWKKLLISEN
FVSPKGFNSLNLKLSQKYPKLSQQDRLRLSFLENIFILKNWYNPKFVPQR
TTLRGHMTSVITCLQFEDNYVITGADDKMIRVYDSINKKFLLQLSGHDGG
VWALKYAHGGILVSGSTDRTVRVWDIKKGCCTHVFKGHNSTVRCLDIVEY
KNIKYIVTGSRDNTLHVWKLPKESSVPDHGEEHDYPLVFHTPEENPYFVG
VLRGHMASVRTVSGHGNIVVSGSYDNTLIVWDVAQMKCLYILSGHTDRIY
STIYDHERKRCISASMDTTIRIWDLENIWNNGECSYATNSASPCAKILGA
MYTLQGHTALVGLLRLSDKFLVSAAADGSIRGWDANDYSRKFSYHHTNLS
AITTFYVSDNILVSGSENQFNIYNLRSGKLVHANILKDADQIWSVNFKGK
TLVAAVEKDGQSFLEILDFSKASKINYVSNPVNSSSSSLESISTSLGLTR
TTIIP*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20561
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of yeast CDC4 N-terminally tagged by StrepII.6HIS.ubiquilin TUBE.; Parental Plasmid SC20434; Estimated MW of expressed protein : 114436 Daltons