cDNA Clone Ubiquitin

• Plasmid: pET156P-Ub2 FRET donor
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-Ubiquitin 1-75 K48R K63R G75D
• Genbank: P62988
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGATTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMQIFVKTLTGKTITLEVEPSDTIENVK
AKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20517
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express 6HIS- Ubiquitin 1-75 K48R K63R G75D (contains preScission protease site); ; Estimated MW of expressed protein : 11067 Daltons