cDNA Clone Ubiquitin

• Synonyms: -
• Plasmid: pET15b-TEV-DiUbiquitin
• Parent Plasmid: pET15b 6His TEV1
• Expressed: 6His-Ubiquitin dimer
• Genbank: P62988*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCAAGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCTTCG
TGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGAC
ACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCC
TGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCA
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTC
CGTCTCAGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MGSSHHHHHHSSGENLYFQGHMLEGSKMQIFVKTLTGKTITLEVEPSDTI
ENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRL
RGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAG
KQLEDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b-TEV-DiUbiquitin1.62 MBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20503
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *A single lysine residue lies N-terminal to the initiating Methionine of the 1st ubiquitin unit; *after subcloning, mutagenic oligos NW415/NW416 were used to correct frame; Bacterial expression construct to express wild type ubiquitin dimer with N-terminal 6HIS tag (contains TEV protease site). Also N-terminal lysine for tryptic cleavage; ; Estimated MW of expressed protein : 20163 Daltons
• Low Temp: No