cDNA Clone Ubiquitin

• Plasmid: pcDNA5-FRT/TO-GS Ubiquitin KallR
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: GS-Ubiquitin KallR
• Genbank: P62988
• Species: Human

Sequence of Insert

AAGCTTGCCACCATGGGCACCCCCGCAGTCACCACCTACAAGCTGGTCAT
TAACGGCAAGACTCTGAAGGGCGAGACCACCACCAAGGCCGTGGACGCAG
AAACCGCGGAAAAGGCGTTCAAGCAGTACGCGAACGACAACGGGGTGGAC
GGAGTCTGGACCTACGATGACGCTACAAAAACGTTCACCGTGACCGAGGT
TAACACCCCCGCTGTGACTACGTACAAGCTGGTGATCAATGGGAAGACCC
TGAAGGGCGAGACCACCACGAAAGCTGTAGACGCCGAGACAGCCGAGAAG
GCCTTCAAGCAGTACGCCAATGACAACGGCGTGGATGGCGTGTGGACCTA
TGACGACGCCACGAAGACATTCACTGTGACTGAGATCGATGAGAACCTCT
ACTTCCAGGGCGGCAGTATGGACGAGAAGACCACCGGCTGGAGAGGCGGC
CACGTGGTCGAGGGACTGGCCGGTGAGCTGGAGCAGCTACGGGCCCGCCT
TGAGCACCACCCCCAGGGGCAGCGCGAGCCGGGCGGAGGCGGAGGCGGAT
CC

Amino Acid Sequence

MGTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWT
YDDATKTFTVTEVNTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQ
YANDNGVDGVWTYDDATKTFTVTEIDENLYFQGGSMDEKTTGWRGGHVVE
GLAGELEQLRARLEHHPQGQREPGGGGGGSMQIFVRTLTGRTITLEVEPS
DTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG**AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20502
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Swapped 6HIS-Gly linker Hind-Bam fragment of SC20446 for GS TAP tag-Gly linker.; NB. Since KallR mutant has internal BamHI site at nt91 subsequently restored full-length by subcloning BamHI flanked 91bp fragment from SC20447 to create final construct.; Flp-In vector for mammalian expression of N-terminally GS TAP tagged KallR ubiquitin (with initiating methionine); SC20447; ; Estimated MW of expressed protein : 27910 Daltons