cDNA Clone UBE2V1

• Plasmid: pET156P-1-UBE2V1
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2V1
• Genbank: AY008273
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCAGGAGAGGTTCAAGCGTCTTACCTGAAGTCACAAAGCAAACT
GAGTGATGAAGGAAGACTTGAACCTAGAAAATTTCACTGCAAAGGAGTAA
AAGTCCCTCGCAATTTCCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAA
GGAGTAGGAGATGGCACAGTTAGCTGGGGTCTAGAAGATGACGAAGACAT
GACACTTACAAGATGGACAGGGATGATAATTGGGCCTCCAAGAACAATTT
ATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCA
GAAGCACCCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGT
AAATAGTTCTAATGGAGTGGTGGACCCAAGAGCCATATCAGTGCTAGCAA
AATGGCAGAATTCATATAGCATCAAAGTTGTCCTGCAAGAGCTTCGGCGC
CTAATGATGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACA
GTGTTACAGCAATTAAGTCGAC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPGEVQASYLKSQSKLSDEGRLEPRKFH
CKGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGP
PRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAI
SVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20496
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2V1 with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 21621 Daltons