cDNA Clone Ubiquitin

• Plasmid: pcDNA5-FRT/TO-GS Ubiquitin
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: GS-Ubiquitin
• Genbank: P62988
• Species: Human

Sequence of Insert

AAGCTTGCCACCATGGGCACCCCCGCAGTCACCACCTACAAGCTGGTCAT
TAACGGCAAGACTCTGAAGGGCGAGACCACCACCAAGGCCGTGGACGCAG
AAACCGCGGAAAAGGCGTTCAAGCAGTACGCGAACGACAACGGGGTGGAC
GGAGTCTGGACCTACGATGACGCTACAAAAACGTTCACCGTGACCGAGGT
TAACACCCCCGCTGTGACTACGTACAAGCTGGTGATCAATGGGAAGACCC
TGAAGGGCGAGACCACCACGAAAGCTGTAGACGCCGAGACAGCCGAGAAG
GCCTTCAAGCAGTACGCCAATGACAACGGCGTGGATGGCGTGTGGACCTA
TGACGACGCCACGAAGACATTCACTGTGACTGAGATCGATGAGAACCTCT
ACTTCCAGGGCGGCAGTATGGACGAGAAGACCACCGGCTGGAGAGGCGGC
CACGTGGTCGAGGGACTGGCCGGTGAGCTGGAGCAGCTACGGGCCCGCCT
TGAGCACCACCCCCAGGGGCAGCGCGAGCCGGGCGGAGGCGGAGGCGGAT
CC

Amino Acid Sequence

MGTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWT
YDDATKTFTVTEVNTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQ
YANDNGVDGVWTYDDATKTFTVTEIDENLYFQGGSMDEKTTGWRGGHVVE
GLAGELEQLRARLEHHPQGQREPGGGGGGSMQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV
LRLRGG**

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20484
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Swapped 6HIS-Gly linker Hind-Bam fragment of SC20446 for GS TAP tag-Gly linker; Flp-In vector for mammalian expression of N-terminally GS TAP tagged wild type ubiquitin (with initiating methionine); SC20446; Estimated MW of expressed protein : 27910 Daltons