cDNA Clone Triad1

• Synonyms: ARIH2
• Plasmid: pGEX6P1-Triad1 C257A
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Triad1 C257A
• Genbank: NM_006321
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA
GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACC
CTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAG
CAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGAC
CTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTT
CTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTC
CACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA
ACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCA
CATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAG
GAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTG
GGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCT
CTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTT
CCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTT
CAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAG
ACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAG
TGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCA
CGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAG
ACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCC
AAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATG
CTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGA
CTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGAC
ATCGTGAACCAGGCCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTA
CTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAG
AGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATG
AACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAA
GCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCAT
ATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCT
CAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGA
CAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGC
GGAGGAGAACCCTGCTGAAAGATTTCCATGACACCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGALNEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQAQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20474
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-Triad1 S353A; ; Estimated MW of expressed protein : 84644 Daltons