cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-Ubiquitin dimer (wild type)
• Parent Plasmid: pGEX6P1
• Expressed: GST-Ubiquitin dimer
• Genbank: P62988*
• Tag: GST
• Species: Human

Sequence of Insert

DNA sequence of insert
GCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCT
CGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAG
ACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAA
CAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTC
CACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAAGGATCCGCGG
CCGC

DNA sequence of entire diubiquitin clone
GGATCCAAGATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCAC
TCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCC
AAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGG
AAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGA
GTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCTTCG
TGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGAC
ACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCC
TGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCA
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTC
CGTCTCAGAGGTGGGTGATAAGGATCCGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSKMQIFVKTLTGKTITLEVE
PSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH
LVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQR
LIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20450
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *pGEX vector backbone has Sfo site deleted; Bacterial construct for expression of GST-ubiquitin (wild type) dimer ; Estimated MW of expressed protein : 44064 Daltons