cDNA Clone DCUN1D3

• Plasmid: pcDNA5-FRT/TO-GFP DCUN1D3
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-DCUN1D3
• Genbank: NM_173475*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGGCCAGTGTGTCACCAAGTGTAAGAATCCCTCATCGACCCT
GGGCAGCAAAAATGGAGACCGTGAGCCCAGCAACAAGTCACATAGCAGGA
GGGGTGCAGGCCACCGTGAGGAGCAGGTACCACCCTGTGGCAAGCCAGGT
GGAGATATCCTCGTCAACGGGACCAAGAAGGCCGAGGCTGCCACTGAGGC
CTGCCAGCTGCCAACGTCCTCGGGAGATGCTGGGAGGGAGTCCAAGTCCA
ATGCCGAGGAGTCTTCCTTGCAAAGATTGGAAGAACTGTTCAGGCGCTAC
AAGGATGAGCGGGAAGATGCAATTTTGGAGGAAGGCATGGAGCGCTTTTG
CAATGACCTGTGTGTTGACCCCACAGAATTTCGAGTGCTGCTCTTGGCTT
GGAAGTTCCAGGCTGCAACCATGTGCAAATTCACCAGGAAGGAGTTTTTT
GATGGCTGCAAAGCAATAAGTGCAGACAGCATTGACGGAATCTGTGCACG
GTTCCCTAGCCTCTTAACAGAAGCCAAACAAGAGGATAAATTCAAGGATC
TCTACCGGTTTACATTTCAGTTTGGCCTGGACTCTGAAGAAGGGCAGCGG
TCACTGCATCGGGAAATAGCCATTGCCCTGTGGAAACTAGTCTTTACCCA
GAACAATCCTCCGGTATTGGACCAATGGCTAAACTTCCTAACAGAGAACC
CCTCGGGGATCAAGGGCATCTCCCGGGACACTTGGAACATGTTCCTTAAC
TTCACTCAGGTGATTGGCCCTGACCTCAGCAACTACAGTGAAGATGAGGC
CTGGCCAAGTCTCTTTGACACCTTTGTGGAGTGGGAAATGGAGCGAAGGA
AAAGAGAAGGGGAAGGGAGAGGTGCACTCAGCTCAGGGCCTGAGGGCTTG
TGTCCCGAGGAGCAGACTTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMGQCVT
KCKNPSSTLGSKNGDREPSNKSHSRRGAGHREEQVPPCGKPGGDILVNGT
KKAEAATEACQLPTSSGDAGRESKSNAEESSLQRLEELFRRYKDEREDAI
LEEGMERFCNDLCVDPTEFRVLLLAWKFQAATMCKFTRKEFFDGCKAISA
DSIDGICARFPSLLTEAKQEDKFKDLYRFTFQFGLDSEEGQRSLHREIAI
ALWKLVFTQNNPPVLDQWLNFLTENPSGIKGISRDTWNMFLNFTQVIGPD
LSNYSEDEAWPSLFDTFVEWEMERRKREGEGRGALSSGPEGLCPEEQT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20442
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *carries a94c mutation (silent Arg32); N terminal GFP tagged DCUN1D3 for mammalian expression (Flp-In vector); ; Estimated MW of expressed protein : 61617 Daltons