cDNA Clone Rsp5

• Plasmid: pRS413-Gal1 Pr-StrepII.6His.TUBE-Rsp5
• Parent Plasmid: pRS413
• Expressed: StrepII.6His.TUBE-Rsp5
• Genbank: 856862
• Tag: 6His
• Species: Saccharomyces cerevisiae

Sequence of Insert

GAATTCATGCCTTCATCCATATCCGTCAAGTTAGTGGCTGCAGAGTCATT
ATATAAGAGGGACGTATTCCGTTCCCCGGATCCGTTTGCTGTTCTGACTA
TTGATGGTTACCAAACCAAATCTACATCCGCAGCGAAGAAAACGTTAAAT
CCCTACTGGAATGAGACTTTCAAATTCGACGATATCAATGAAAATTCTAT
TTTAACCATCCAAGTCTTTGATCAAAAGAAATTTAAAAAGAAGGATCAAG
GGTTTCTTGGCGTGGTTAACGTCCGCGTGGGTGATGTTTTGGGCCATTTG
GATGAAGATACTGCTACATCGAGTGGTAGACCTCGCGAAGAAACTATTAC
TCGTGATTTAAAAAAATCTAATGACGGAATGGCCGTCAGCGGTAGGTTGA
TTGTTGTTTTATCAAAACTACCTTCGTCAAGTCCGCATTCACAAGCTCCT
TCTGGCCATACTGCATCTTCTAGTACGAATACAAGTTCAACTACTCGAAC
AAATGGTCATTCTACTTCCTCCACCCGTAATCATTCAACGTCTCATCCTT
CCAGAGGCACCGCGCAAGCAGTAGAATCAACTCTTCAAAGCGGTACAACA
GCTGCTACTAATACGGCAACAACCAGTCACCGTTCCACCAATTCCACATC
CAGTGCTACCAGACAATACTCTTCGTTTGAAGACCAGTATGGTCGTTTAC
CCCCTGGTTGGGAGAGAAGGACCGATAACTTTGGTCGTACATATTACGTC
GATCATAACACAAGGACTACCACTTGGAAACGTCCAACGCTCGATCAAAC
AGAAGCCGAACGTGGCAACCAATTAAATGCAAATACTGAGTTAGAGAGAA
GGCAGCATAGGGGAAGAACTTTACCTGGTGGATCATCAGATAATTCCTCT
GTAACAGTTCAAGTGGGAGGTGGTTCCAATATACCTCCTGTTAATGGTGC
AGCAGCAGCAGCGTTTGCAGCTACAGGTGGTACCACATCTGGTTTAGGCG
AATTACCTTCAGGCTGGGAGCAGCGATTTACTCCAGAAGGAAGAGCTTAT
TTCGTTGACCATAATACTAGAACAACCACTTGGGTGGATCCAAGGAGACA
ACAGTATATACGTACTTATGGCCCTACAAATACCACCATTCAGCAACAAC
CGGTCTCCCAATTAGGTCCTTTGCCTTCTGGATGGGAAATGAGATTGACC
AATACGGCACGTGTATATTTCGTTGACCACAATACAAAAACAACGACCTG
GGATGACCCAAGACTTCCATCATCGCTAGACCAAAATGTTCCACAATACA
AGCGTGACTTCAGACGTAAGGTTATTTATTTCAGGTCCCAGCCCGCTCTT
AGAATATTGCCGGGACAATGTCATATTAAAGTACGTAGAAAGAACATTTT
TGAGGATGCTTATCAAGAAATTATGAGACAAACCCCAGAAGATTTAAAGA
AAAGATTAATGATCAAGTTTGATGGTGAGGAAGGTTTAGATTACGGTGGT
GTTTCCAGAGAATTTTTCTTTTTATTATCACATGAGATGTTTAATCCATT
TTATTGTTTGTTTGAATATTCTGCGTATGACAACTATACCATTCAAATAA
ATCCTAACAGTGGCATCAACCCCGAACATTTGAACTATTTCAAATTCATT
GGTAGAGTTGTGGGTCTTGGTGTTTTCCATAGAAGATTTTTGGATGCATT
CTTTGTAGGTGCACTTTACAAAATGATGCTACGTAAAAAAGTCGTATTGC
AAGATATGGAAGGTGTTGACGCAGAGGTGTATAACTCATTAAACTGGATG
CTTGAGAATAGTATAGATGGTGTCTTGGATCTGACGTTCAGTGCCGACGA
TGAAAGATTCGGTGAAGTTGTAACAGTGGATTTGAAGCCAGATGGGAGAA
ATATTGAAGTTACTGATGGTAATAAGAAAGAATATGTCGAATTATATACC
CAATGGAGAATTGTTGATAGAGTTCAAGAACAATTTAAGGCATTCATGGA
TGGTTTTAACGAATTAATCCCCGAAGACTTAGTAACTGTGTTTGATGAGC
GTGAACTAGAACTATTGATCGGTGGTATTGCGGAAATTGACATTGAAGAT
TGGAAGAAACACACTGATTATCGTGGTTACCAAGAGTCAGACGAGGTCAT
TCAATGGTTTTGGAAGTGTGTCAGTGAATGGGATAATGAACAAAGAGCCC
GTTTATTGCAGTTCACCACTGGTACTTCTCGTATACCTGTCAACGGGTTT
AAAGATTTGCAAGGCTCTGATGGTCCAAGAAGATTCACTATTGAAAAAGC
TGGTGAAGTACAACAATTGCCAAAATCTCACACATGTTTTAACAGAGTTG
ATTTGCCACAATACGTTGATTATGACAGCATGAAACAGAAGCTAACATTG
GCCGTGGAAGAAACCATAGGGTTTGGTCAAGAATGAGCGGCCGC

Amino Acid Sequence

MWSHPQFEKGAHHHHHHRSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNRE
ANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQL
EQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQ
LQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS
GGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDIN
AAIERLLGSQPSGGGGSGGGGGGGGSPEFMPSSISVKLVAAESLYKRDVF
RSPDPFAVLTIDGYQTKSTSAAKKTLNPYWNETFKFDDINENSILTIQVF
DQKKFKKKDQGFLGVVNVRVGDVLGHLDEDTATSSGRPREETITRDLKKS
NDGMAVSGRLIVVLSKLPSSSPHSQAPSGHTASSSTNTSSTTRTNGHSTS
STRNHSTSHPSRGTAQAVESTLQSGTTAATNTATTSHRSTNSTSSATRQY
SSFEDQYGRLPPGWERRTDNFGRTYYVDHNTRTTTWKRPTLDQTEAERGN
QLNANTELERRQHRGRTLPGGSSDNSSVTVQVGGGSNIPPVNGAAAAAFA
ATGGTTSGLGELPSGWEQRFTPEGRAYFVDHNTRTTTWVDPRRQQYIRTY
GPTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLP
SSLDQNVPQYKRDFRRKVIYFRSQPALRILPGQCHIKVRRKNIFEDAYQE
IMRQTPEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEY
SAYDNYTIQINPNSGINPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALY
KMMLRKKVVLQDMEGVDAEVYNSLNWMLENSIDGVLDLTFSADDERFGEV
VTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELI
PEDLVTVFDERELELLIGGIAEIDIEDWKKHTDYRGYQESDEVIQWFWKC
VSEWDNEQRARLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIEKAGEVQQL
PKSHTCFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQE*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20435
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of Rsp5 N-terminally tagged by StrepII.6HIS.ubiquilin TUBE. ; Parental Plasmid SC20406; Estimated MW of expressed protein : v Daltons