cDNA Clone Triad1

• Synonyms: ARIH2
• Plasmid: pFBHT-Triad1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Triad1
• Genbank: NM_006321
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA
GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACC
CTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAG
CAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGAC
CTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTT
CTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTC
CACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA
ACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCA
CATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAG
GAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTG
GGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCT
CTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTT
CCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTT
CAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAG
ACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAG
TGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCA
CGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAG
ACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCC
AAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATG
CTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGA
CTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGAC
ATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTA
CTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAG
AGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATG
AACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAA
GCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCAT
ATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCT
CAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGA
CAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGC
GGAGGAGAACCCTGCTGAAAGATTTCCATGACACCTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMSVDMNSQGSDSNEEDYDPNCE
EEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGA
LNEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARV
QPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQFCRSCWEQHCSVL
VKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESH
YQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCAT
IRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDF
CWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERW
ENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRY
TLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDL
ENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20432
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression construct for 6HIS-Triad1 (Drosophila Ariadne homolog 2); ; Estimated MW of expressed protein : 61191 Daltons