cDNA Clone TRAFD1

• Plasmid: pEX-GST-TRAFD1
• Parent Plasmid: pEX C-GST
• Expressed: TRAFD1-GST
• Genbank: NM_006700.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAAGGAGATGGCTGAATTTCTAGATGACCAGGAAACTCGACTGTGTGACAACTGCAAAAAAGAAATTCCTGTGTTTAACTTTACCATCCATGAGATCCACTGTCAAAGGAACATTGGTATGTGTCCTACCTGTAAGGAACCATTTCCCAAATCTGACATGGAGACTCACATGGCTGCAGAACACTGTCAGGTGACCTGCAAATGTAACAAGAAGTTGGAGAAGAGGCTGTTAAAGAAGCATGAGGAGACTGAGTGCCCTTTGCGGCTTGCTGTCTGCCAGCACTGTGATTTAGAACTTTCC
ATTCTCAAACTGAAGGAACATGAAGATTATTGTGGTGCCCGGACGGAACT
ATGTGGCAACTGTGGTCGCAATGTCCTTGTGAAAGATCTGAAGACTCACC
CTGAAGTTTGTGGGAGAGAGGGGGAGGAAAAGAGAAATGAGGTTGCCATA
CCTCCTAATGCATATGATGAATCTTGGGGTCAGGATGGAATCTGGATTGC
ATCCCAACTCCTCAGACAAATTGAGGCTCTGGACCCACCCATGAGGCTGC
CGCGAAGGCCCCTGAGAGCCTTTGAATCAGATGTTTTCCACAATAGAACT
ACCAACCAAAGGAACATTACAGCCCAGGTTTCAATTCAGAATAATCTGTT
TGAAGAACAAGAGAGGCAGGAAAGGAATAGAGGCCAACAGCCCCCCAAAG
AGGGTGGTGAAGAGAGTGCAAACTTGGACTTCATGTTGGCCCTAAGTCTG
CAAAATGAAGGCCAAGCCTCCAGTGTGGCAGAGCAGGACTTCTGGAGGGC
CGTATGTGAGGCCGACCAGTCTCATGGCGGTCCCAGGTCTCTCAGTGACA
TAAAGGGTGCAGCTGACGAGATCATGTTGCCTTGTGAATTTTGTGAGGAG
CTCTACCCAGAGGAACTGCTGATTGACCATCAGACAAGCTGTAACCCTTC
ACGTGCCTTACCTTCACTCAATACTGGCAGCTCTTCCCCCAGAGGGGTGG
AGGAACCTGATGTCATCTTCCAGAACTTCTTGCAACAGGCTGCAAGTAAC
CAGTTAGACTCTTTGATGGGCCTGAGCAATTCACACCCTGTGGAGGAGAG
CATCATTATCCCATGTGAATTCTGTGGGGTACAGCTGGAAGAGGAGGTGC
TGTTCCATCACCAGGACCAGTGTGACCAACGCCCAGCCACTGCAACCAAC
CATGTGACAGAGGGGATTCCTAGACTGGATTCCCAGCCTCAAGAGACCTC
ACCAGAGCTGCCCAGGAGGCGTGTCAGACACCAGGGAGACCTGTCTTCTG
GTTACCTGGATGATACTAAGCAGGAAACAGCTAATGGGCCCACCTCCTGT
CTGCCTCCCAGCCGACCCATTAACAATATGACAGCTACCTATAACCAGCT
ATCGAGATCAACATCAGGCCCCAGACCTGGGTGCCAGCCCAGCTCTCCTT
GTGTGCCGAAGCTCAGCAACTCAGACAGCCAGGACATCCAGGGGCGGAAT
CGAGACAGCCAGAATGGGGCCATAGCCCCTGGGCACGTTTCAGTGATTCG
CCCTCCTCAAAATCTCTACCCAGAAAACATTGTGCCCTCTTTCTCCCCTG
GGCCTTCAGGGAGATACGGAGCTAGTGGTAGGAGTGAAGGTGGCAGGAAT
TCCCGGGTCACCCCTGCAGCTGCCAACTACCGCAGCAGAACTGCAAAGGC
AAAGCCTTCCAAGCAACAGGGAGCTGGGGATGCAGAAGAGGAAGAGGAGG
AGGCGGCCGC

Amino Acid Sequence

MAEFLDDQETRLCDNCKKEIPVFNFTIHEIHCQRNIGMCPTCKEPFPKSD
METHMAAEHCQVTCKCNKKLEKRLLKKHEETECPLRLAVCQHCDLELSIL
KLKEHEDYCGARTELCGNCGRNVLVKDLKTHPEVCGREGEEKRNEVAIPP
NAYDESWGQDGIWIASQLLRQIEALDPPMRLPRRPLRAFESDVFHNRTTN
QRNITAQVSIQNNLFEEQERQERNRGQQPPKEGGEESANLDFMLALSLQN
EGQASSVAEQDFWRAVCEADQSHGGPRSLSDIKGAADEIMLPCEFCEELY
PEELLIDHQTSCNPSRALPSLNTGSSSPRGVEEPDVIFQNFLQQAASNQL
DSLMGLSNSHPVEESIIIPCEFCGVQLEEEVLFHHQDQCDQRPATATNHV
TEGIPRLDSQPQETSPELPRRRVRHQGDLSSGYLDDTKQETANGPTSCLP
PSRPINNMTATYNQLSRSTSGPRPGCQPSSPCVPKLSNSDSQDIQGRNRD
SQNGAIAPGHVSVIRPPQNLYPENIVPSFSPGPSGRYGASGRSEGGRNSR
VTPAAANYRSRTAKAKPSKQQGAGDAEEEEEEAAALEVLFQGPLSRMSPI
LGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPN
LPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFM
LYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQ
GWQATFGGGDHPPKSDL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20407
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for TRAFD1-GST; ; Estimated MW of expressed protein : 92092 Daltons