cDNA Clone UBE2V1

• Synonyms: TRAF6-regulated IKK activator 1 beta Uev1A
• Plasmid: pET28a-UBE2V1
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2V1
• Genbank: AY008273
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCAGGAGAGGTTCAAGCGTCTTACCTGAAGTCACAAAGCAAACT
GAGTGATGAAGGAAGACTTGAACCTAGAAAATTTCACTGCAAAGGAGTAA
AAGTCCCTCGCAATTTCCGACTGTTGGAAGAACTCGAAGAAGGCCAGAAA
GGAGTAGGAGATGGCACAGTTAGCTGGGGTCTAGAAGATGACGAAGACAT
GACACTTACAAGATGGACAGGGATGATAATTGGGCCTCCAAGAACAATTT
ATGAAAACCGAATATACAGCCTTAAAATAGAATGTGGACCTAAATACCCA
GAAGCACCCCCCTTTGTAAGATTTGTAACAAAAATTAATATGAATGGAGT
AAATAGTTCTAATGGAGTGGTGGACCCAAGAGCCATATCAGTGCTAGCAA
AATGGCAGAATTCATATAGCATCAAAGTTGTCCTGCAAGAGCTTCGGCGC
CTAATGATGTCTAAAGAAAATATGAAACTCCCTCAGCCGCCCGAAGGACA
GTGTTACAGCAATTAAGTCGAC

Amino Acid Sequence

HHHHHHSSGLVPRGSHMASMTGGQQMGRGSPGEVQASYLKSQSKLSDEGR
LEPRKFHCKGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRW
TGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNG
VVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQCYSN*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28a-UBE2V158.44 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20179
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2V1 with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 22358 Daltons
• Low Temp: No