cDNA Clone UBE2Q

• Synonyms: -
• Plasmid: pET28-UBE2Q
• Parent Plasmid: pET28a original
• Expressed: 6His-UBE2Q
• Genbank: NP_060052
• Tag: 6His
• Species: Human

Sequence of Insert

GAATTCCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCA
GCAGCTGGGGGGCCAGGGGGCGGCGCCGGGGGCCGGGGGCGGCCCAGGGG
GGGGCCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCTGCTCGAG
TCCATCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCT
GGACGAGCTGAGCTGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGG
CGGGGGCCGCGCCCGGACCGCATCTCCCCCCACGGGGGTCGGTGCCTGGG
GATCCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCC
CCCCATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGG
AGAGGCTGGTGGACATAAAGAAAGGGAATACTCTGCTATTGCAGCATCTG
AAGAGGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCTCAGCATCC
AGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGG
AAGACGTGTCTTCAGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAA
GACTTAGATCACTATGAAATGAAAGAGGAAGAGCCAGCTGAGGGCAAGAA
ATCTGAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAA
TTAAAAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCG
GTGCAGGCCACTGACCGGCTGATGAAGGAGCTCAGGGATATATACCGATC
ACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAATGACAGTC
TGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTG
CACAACGATCTCCAGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCT
ACTTAACTTTTCCTTTAAAGATAACTTTCCCTTTGACCCACCATTTGTCA
GGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCC
ATCTGCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCAT
AGAGTCAGTGATCATGCAGATCAGTGCCACACTGGTGAAGGGGAAAGCAC
GAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAGAGCACAG
CAGTCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACAC
ACCCCCAAAAGAAGACGGCTAAGCGGCCGC

Amino Acid Sequence

HHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFQQPQPQGQQQPGPGQQLG
GQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLDEL
SCEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPPIW
SVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQHPDVE
MLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKSED
DGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQSF
KGGNYAVELVNDSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILLNF
SFKDNFPFDPPFVRVVSPVLSGGYVLGGGAICMELLTKQGWSSAYSIESV
IMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPK
EDG*

• Vector Type: Bacterial
• Full Snapgene Map: pET28-UBE2Q105.26 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20176
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express UBE2Q with N-terminal 6HIS tag (contains Thrombin cleavage site); ; Estimated MW of expressed protein : 49454 Daltons
• Low Temp: No