cDNA Clone Triad1

• Plasmid: pGEX6P-1 Triad1 340-493
• Parent Plasmid: pGEX6P1
• Expressed: GST-Triad1 340-493
• Genbank: NM_006321*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTGCAGTCGTTACAAGGAGAATCCTGACATCGTGAACCAGAGCCA
ACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACTTATTCTACTTTGAGA
GGTGGGAAAACCACAATAAAAGCTTGCAGCTAGAGGCACAGACATACCAG
CGGATTCACGAGAAGATTCAGGAGAGGGTCATGAACAATCTGGGGACATG
GATCGACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTGGCCAAGTGTC
GATACACCCTGCAATACACCTACCCATATGCATATTACATGGAGTCCGGA
CCCAGGAAGAAGCTGTTTGAATACCAGCAGGCTCAGCTGGAGGCTGAGAT
CGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACAGCTATGACAGAGGGG
ACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAGAACCCTGCTG
AAAGATTTCCATGACACCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSCSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Bacterial
• DU Number: DU20159
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- Triad1 aa340-493.; Clone contains nt 1018 – 1482 of the reference sequence plus artificial STOP codon.; ; Estimated MW of expressed protein : 45668 Daltons