cDNA Clone Triad1

• Plasmid: pGEX6P-1 Triad1 120-493
• Parent Plasmid: pGEX6P1
• Expressed: GST-Triad1 120-493
• Genbank: NM_006321*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCACATCCCA
TCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAGGAAAACC
TACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTGGGAGCAG
CACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCTCTTGCAT
GGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTTCCATTGC
TTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTTCAGGGAC
TATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAGACTGCCC
CATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAGTGCAATC
GGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCACGCACCC
ACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAGACGACTC
TGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCA
ACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATGCTCCAAA
TGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGACTCATGG
CAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGACATCGTGA
ACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACTTATTC
TACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAGAGGCACA
GACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATGAACAATC
TGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTG
GCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCATATTACAT
GGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCTCAGCTGG
AGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACAGCTAT
GACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAG
AACCCTGCTGAAAGATTTCCATGACACCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Bacterial
• DU Number: DU20156
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST- Triad1 aa120-493.; Clone contains nt 358 – 1482 of the reference sequence plus artificial STOP codon.; ; Estimated MW of expressed protein : 71210 Daltons