cDNA Clone Ubiquitin

• Plasmid: pGEX6P1-Ubiquitin G75A G76A
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-Ubiquitin G75A G76A
• Genbank: P62988
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGGCGCCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGCTGCTTGATAAGCGG
CCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGAMQIFVKTLTGKTITLEV
EPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTL
HLVLRLRAA**

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20124
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Source of C-terminal unit for polyubiquitin chain assembly. ; Bam-Not fragment harbouring C-terminal single ubiquitin unit with C-terminal AA instead of GG and STOP codon. SfoI site just downstream of BamHI site enables progressive 5’ assembly of chain units.; pGEX vector backbone has Sfo site deleted DUSC20103; ; Estimated MW of expressed protein : 35546 Daltons