cDNA Clone Ubiquilin

• Plasmid: pEBG6P-1-UBIQUILIN TUBE
• Parent Plasmid: pEBG6P
• Expressed: GST-ubiquilin TUBE
• Genbank: NM_053067.2
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATT
TCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAG
CAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATT
GAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGG
AGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGG
AACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCT
CTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGG
CTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGC
TACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCA
ATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGG
AGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAG
GAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAA
GTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAA
CCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATG
CAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCATAGGGAGGTGGAGGA
TCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSGGGVNPQLQNPEV
RFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSG
GGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERL
LGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIA
TGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGF
LNREANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20109
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Ubiquilin-based TUBE with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 52573 Daltons