cDNA Clone Ubiquilin

• Plasmid: pET156P-1-UBIQUILIN TUBE
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-ubiquilin TUBE
• Genbank: NM_053067.2
• Tag: 6His
• Species: Human

Sequence of Insert

AGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATT
TCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAG
CAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATT
GAAAGGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGG
AGTAAATCCTCAGCTACAGAATCCAGAAGTCAGATTTCAGCAACAACTGG
AACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCT
CTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGG
CTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGC
TACAGAATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCA
ATGGGATTTTTGAACCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGG
AGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCAG
GAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAA
GTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAA
CCGTGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATG
CAGCTATTGAAAGGTTACTGGGCTCCCAGCCATCATAGGGAGGTGGAGGA
TCC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSGGGVNPQLQNPEVRFQQQLEQLSAMGF
LNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGGVNPQLQNPEVRF
QQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSGGGGSGGG
VNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLG
SQPSGGGGSGGGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATG
GDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct to express Ubiquilin-based TUBE with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 27584 Daltons