cDNA Clone Triad1

• Synonyms: ARIH2
• Plasmid: pcDNA5-FRT/TO-GFP Triad1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-Triad1
• Genbank: NM_006321
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGA
GGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACC
CTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAG
CAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGAC
CTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTT
CTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTC
CACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCA
ACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCA
CATCCCATCCCCCTCACCACTGTGCAGTGTGTATGCAGTTTGTGCGAAAG
GAAAACCTACTCTCTCTGGCCTGTCAGCACCAGTTTTGCCGCAGCTGCTG
GGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCT
CTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTT
CCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTT
CAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAG
ACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAG
TGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCA
CGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAG
ACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCC
AAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATG
CTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGA
CTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGAC
ATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTA
CTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAG
AGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATG
AACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAA
GCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCAT
ATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCT
CAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGA
CAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGC
GGAGGAGAACCCTGCTGAAAGATTTCCATGACACCTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSVDMN
SQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDP
EEYQFTCLTYKESEGALNEHMTSLASVLKVSHSVAKLILVNFHWQVSEIL
DRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCAVCMQFVRKENLLSLAC
QHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEEL
REKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVF
CFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEK
NGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQA
QAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWID
WQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIEN
LSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20095
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP for mammalian expression Flp-In vector.; ; Estimated MW of expressed protein : 85145 Daltons