cDNA Clone UBE2U

• Plasmid: pGEX6P-1-UBE2U
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBE2U
• Genbank: Q5VVX9
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCACGGCAGAGCTTACCTCTTGCTGCACAGAGACTTCTGTGA
TCTCAAGGAGAACAATTATAAGGGTATCACTGCTAAGCCTGTAAGTGAAG
ATATGATGGAATGGGAAGTTGAAATTGAAGGTCTACAGAATTCAGTTTGG
CAGGGTTTAGTCTTCCAACTGACAATACATTTTACATCGGAGTACAACTA
TGCTCCTCCAGTTGTGAAATTTATAACAATTCCGTTTCATCCAAATGTAG
ACCCACACACTGGTCAGCCCTGTATAGACTTTTTGGACAACCCTGAGAAG
TGGAATACAAACTATACATTGAGCAGCATCTTACTTGCCCTACAGGTTAT
GCTTTCTAATCCAGTGCTAGAGAATCCAGTGAATTTGGAAGCAGCCAGAA
TACTGGTTAAAGATGAATCTCTGTACAGAACAATTCTAAGACTTTTCAAC
AGGCCATTACAAATGAAAGATGACAGCCAGGAGTTACCTAAAGACCCACG
TAAATGTATCAGACCAATTAAAACAACCTCATTTAGTGATTACTACCAGA
CATGGTCCAGAATAGCTACATCAAAAGCCACAGAATACTACAGAACTCCA
TTGCTCAAAGTTCCAAATTTCATTGGACAGTATTACAAATGGAAGAAAAT
GGATCTACAGCATCAGAAAGAATGGAATTTAAAGTATTCCGTTATCAAGT
GTTGGCTTGCTAGAAAAAGAATGCCTCATGAAGTCACTCACTCAATGGAA
GAAATTAAGCTCTGCCCAACTCTAATTCCGACCACAGATGAAATTTTTCT
TGAGTCACCAACTGCAATAAATAGCATCACAGACATTTATGAAACAGAAG
AGGAGGGGTGGAAGAGTGACACTTCATTATATGAAAATGACACAGATGAG
CCCAGGGAAGAGGAAGTGGAAGATCTGATCTCCTGGACCAATACTCTCAA
TACAAATACTTCAGAAGATTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMHGRAYLLLHRDFCDLKEN
NYKGITAKPVSEDMMEWEVEIEGLQNSVWQGLVFQLTIHFTSEYNYAPPV
VKFITIPFHPNVDPHTGQPCIDFLDNPEKWNTNYTLSSILLALQVMLSNP
VLENPVNLEAARILVKDESLYRTILRLFNRPLQMKDDSQELPKDPRKCIR
PIKTTSFSDYYQTWSRIATSKATEYYRTPLLKVPNFIGQYYKWKKMDLQH
QKEWNLKYSVIKCWLARKRMPHEVTHSMEEIKLCPTLIPTTDEIFLESPT
AINSITDIYETEEEGWKSDTSLYENDTDEPREEEVEDLISWTNTLNTNTS
ED*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20062
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for UBE2U with N-terminal GST tag (contains preScission protease site); ; Estimated MW of expressed protein : 64566 Daltons