cDNA Clone Ubiquitin

• Plasmid: pET24-Ubiquitin c220a K63R mutant
• Parent Plasmid: pET24a original
• Expressed: untagged Ubiquitin c220a mutant K63R
• Genbank: P62988*
• Tag: untagged
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTG
GAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCACCCT
GCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQRESTLHLVLRLRGG

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20029
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for untagged ubiquitin K63R mutant.; Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389) and by K63R substitution conferred by primers MP1758/MP1759; ; Estimated MW of expressed protein : 8593 Daltons