cDNA Clone Ubiquitin

• Plasmid: pET24-Ubiquitin (silent a220c)
• Parent Plasmid: pET24a original
• Expressed: untagged Ubiquitin
• Genbank: P62988*
• Tag: untagged
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET24-Ubiquitin (silent a220c)68.97 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU20027
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for untagged ubiquitin (Arg73 codon AGA mutated to more common CGA); ; Differs from reference sequence in having silent mutation at Arg73 (a220c substitution conferred by reverse primer 54389).; T7 tag in pET24a removed by subcloning into the Nde1 site, to give untagged protein. ; ; Estimated MW of expressed protein : 8565 Daltons
• Low Temp: No