cDNA Clone USP11

• Plasmid: pFBHT-USP11
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-USP11
• Genbank: NM_004651
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCTATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGTTTCCGGGACCAGAATCCGGAAGTGGCTGTTGAGGGGCGTCTTCCAATCTCGCACAGCTGCGTTGGCTGTAGAAGAGAACGGACGGCGATGGCGACGGTCGCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCGGCAGCGGCGGTGACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGCCAGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGGGCCGGCGAAAGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAGGCATACGTGCAGGGAGGGGACCAGGACTCCAGCACCTTCCCTGGCTGCATCAACAATGCCACACTCTTTCAAGATGAGATAAACTGGCGCCTCAAGGAGGGACTGGTG
GAAGGCGAGGATTATGTGCTGCTCCCAGCAGCTGCTTGGCATTACCTGGT
CAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAACGCAAGGTCA
TAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTAGAACTGCTG
CTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCAGTTCAGCCA
TACCGATTCTATTGGCCTAGTATTGCGCACAGCTCGGGAGCGGTTTCTGG
TGGAGCCCCAGGAAGACACTCGGCTTTGGGCCAAGAACTCAGAAGGCTCT
TTGGATAGGTTGTATGACACACACATCACGGTTCTCGATGCGGCCCTTGA
GACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGATGGCACTTGGC
CCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAGAGGATGAG
GACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCTGGGCAACAC
GTGCTTCATGAACTCGGCCCTGCAGTGCCTCAGCAATGTGCCACAGCTCA
CCGAGTACTTCCTCAACAACTGCTACCTGGAGGAGCTCAACTTCCGCAAC
CCACTGGGCATGAAGGGTGAGATCGCAGAGGCCTATGCAGACCTGGTGAA
GCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACATGTGTTCAAGA
ACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAGCAGCATGAC
TCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAGGACCTTAA
TCGGGTGAAGAAGAAGGAGTATGTGGAGCTGTGCGATGCTGCTGGGCGAC
CGGATCAGGAGGTGGCACAGGAGGCATGGCAAAACCACAAACGGCGGAAC
GATTCTGTGATCGTGGACACTTTCCACGGCCTCTTCAAGTCCACGCTGGT
GTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCCTTCTGCTACC
TCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAGGTCTTCTTT
ATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCTCGTGGTCCC
CAAGAAAGGCAAGATCTCGGATCTATGTGTGGCTCTGTCCAAACACACGG
GCATCTCGCCAGAGAGGATGATGGTGGCTGATGTCTTCAGTCACCGCTTC
TATAAGCTCTATCAGCTAGAGGAGCCTCTGAGCAGCATCTTGGACCGTGA
TGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCCATTGAGGGCT
CAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCACCCCTGCC
CGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGGACACCCCCT
CCTGGTATCAGTGCCCCGGGACCGCTTCACCTGGGAGGGCCTGTATAACG
TCCTGATGTACCGGCTCTCACGCTACGTGACCAAACCCAACTCAGATGAT
GAGGACGATGGGGATGAGAAAGAAGATGACGAGGAGGATAAAGATGACGT
CCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAGCCAGAGCAGG
CTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCCTGGACAAT
TGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAGCAGCTGTT
CACCCTGCAGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCC
CTGAAGAAGTCCATGCCCAGCCGTACATTGCTATCGACTGGGAGCCAGAG
ATGAAGAAGCGTTACTATGACGAGGTAGAGGCTGAGGGCTACGTGAAGCA
TGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGGCTGCAGGAGT
GCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAAAACCCCTGG
TACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAGCTGGACCT
GTGGATGCTGCCGGAGATTCTCATCATCCACCTGAAACGCTTTTCCTACA
CCAAGTTCTCCCGAGAGAAGCTGGACACCCTCGTGGAGTTTCCTATCCGG
GACCTGGACTTCTCTGAGTTTGTCATCCAGCCACAGAATGAGTCGAATCC
GGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCATTATGGGGGCA
TGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGACAGCGGCCAG
TGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGAGAATCAGAT
CGAGTCCAAGGCAGCCTATGTCCTCTTCTACCAACGCCAGGACGTGGCGC
GACGCCTGCTGTCCCCGGCCGGCTCATCTGGCGCCCCAGCCTCCCCTGCC
TGCAGCTCCCCACCCAGCTCTGAGTTCATGGATGTTAATTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMAVAPRL
FGGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAA
VAAAAAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEK
HWYKQWEAYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVL
LPAAAWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDL
GKSHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDT
HITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPG
ICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGE
IAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSF
LLDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDT
FHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRR
KPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLE
EPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSY
YGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEK
EDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQW
PPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYD
EVEAEGYVKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQ
HQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEF
VIQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDN
SVSPVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSS
EFMDVN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20021
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express USP11 with N-terminal 6HIS tag (contains TEV protease site); ; Estimated MW of expressed protein : 114630 Daltons