cDNA Clone UBE4a

• Plasmid: pFBHT-UBE4a
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-UBE4a
• Genbank: NM_004788
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTGATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGCTCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTATTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGAAGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCTCGCTTATTACTTCAAGATCCAGGCAACCAC
TTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTGCTGATCGAGA
TGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAG
CCAAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTG
CAGTGCAGAAACCTCACTGTGTCCAATACCCGAACAGTTCTTCTCACCCC
AGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT
TAGAAGCCATCCAGGGAGCCCGTGAGTACATGAACAAGATCTATTTTGAA
GATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGATATTGGATGA
GGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTAT
TGGGCCGAATAAAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATAT
CTGGATATTCTTCTCTATTTCACTAGGCAAAAAGATATGGCAAAGGTTTT
TGTAGAATACATTCAGCCCAAGGACCCTACCAATGGGCAAATGTACCAGA
AGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCG
GGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCC
CCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGT
TCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCA
GAAACCAAACACTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAA
TGCAGGCCGCACCAAGATTTGGGCCAATCAGATGCCAGAAATCTTTTTCC
AAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTG
AAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTT
TAATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAA
TTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGTTTGATCCCA
GCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTGTAACAGAGAA
CCTTGCTCTGACAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATG
ATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAGGTTGCCTGG
CGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCA
GTTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAG
AGCCACAAATGCTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTT
CTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCCAATAGAGCT
AACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAAT
TTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCC
GATGACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTT
TATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGA
GGGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAG
ACCCCAAATCCCTTGGTATCCAGTGTGTTCCACCGGAAACGTGTGTTCTG
CAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCAAGGTTTTTG
TGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAAT
TACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATAC
CTATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAG
AAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAAT
GATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAA
GATTCAGCAAATTGAGAAGGATCGAGGTGAATGGGATAGTCTGACTCCAG
AAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGACAGCTGGCA
CGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCT
CACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCA
TCATCTCCATGTTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATG
GGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCA
GCTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGA
ATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCCCCAACTCTC
TTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAATAT
GATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACC
TCCAACAACAGGAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTG
GATCCCATTATGAGCACACTGATGTGTGACCCTGTGGTGCTGCCATCTTC
CAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACC
AAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCA
AACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACA
ACAAAAGGAGCAACTTGAATAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMTDQENN
NNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSES
LDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGN
GIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSS
TTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTV
SNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLE
EVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYF
TRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHG
YFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCIL
SWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFC
KPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKF
PQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSS
SPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAI
GNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETS
ADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVS
SVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYP
ILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLD
EAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMS
NETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKD
FSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRV
LKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTL
MCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEK
IQRWLAERKQQKEQLE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express Ube4a with N-terminal 6HIS tag (contains TEV protease site); ; Estimated MW of expressed protein : 128335 Daltons