cDNA Clone UBE2B

• Synonyms: HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
• Plasmid: pET156P UBE2B
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C UBE2B
• Genbank: NM_003337
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCG
GTTACAAGAGGACCCACCTGTGGGTGTCAGTGGCGCACCATCTGAAAACA
ACATCATGCAGTGGAATGCAGTTATATTTGGACCAGAAGGGACACCTTTT
GAAGATGGTACTTTTAAACTAGTAATAGAATTTTCTGAAGAATATCCAAA
TAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAATGTGT
ATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCA
ACATATGATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGA
ACCGAATCCTAACAGTCCAGCCAATAGCCAGGCAGCACAGCTTTATCAGG
AAAACAAACGAGAATATGAGAAAAGAGTTTCGGCCATTGTTGAACAAAGC
TGGAATGATTCATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMSTPARRRLMRDFKRLQEDPPVGVSGA
PSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMF
HPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAA
QLYQENKREYEKRVSAIVEQSWNDS

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20017
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express Rad 6 with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 19757 Daltons
• Low Temp: No