cDNA Clone HPK1

• Synonyms: HPK1, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, hematopoietic progenitor kinase 1, MAP4K1
• Plasmid: pCMV5 FLAG HPK1
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG HPK1
• Genbank: NM_001042600.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCG GGACCACTATGACCTGCTACAGCGGCTGGGTGGCGGCACGTATGGGGAAG TCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGATG GTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCT CATATTGAAAACTTGCCGGCACGCCAACATCGTGGCCTACCATGGGAGTT ATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCTGTGGGGCTGGT TCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGAT TAGCTATGTCTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCAC AGAAGAAGATACACAGGGACATCAAGGGAGCTAACATCCTCATCAATGAT GCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGGC TACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTC CGGAAGTGGCAGCTGTGGCCCTGAAGGGAGGATACAATGAGCTGTGTGAC ATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAGCTACAGCCACC GCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTG GCTACCAGCCTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTC CACAACTTCATCAAAGTCACTCTGACTAAGAGTCCCAAGAAACGACCCAG CGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAATC GAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGA CCCTCCATTGGGGACATTGAGGATGAGGAGCCCGAGCTACCCCCTGCTAT CCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCTGGGGATCCCAG ATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATG GAGACCAGACCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCT CAGGAGCAGCAGCCCCAGGAAGCAACTGTCAGAGTCGTCTGACGATGACT ATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACTT CCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCAT GGGGGATGATGGGCAGCTGAGCCCGGGGGTGCTGGTCCGGTGTGCCAGTG GGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCATCCACCAGCAGC CCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCG GGAGCTTGACAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGA GAAAGGGATGTGCCCTTCTCGTAAAGTTGTTCAATGGCTGCCCCCTCCGG ATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACCT GCTCCTGGGGGCAGAGGAAGGCATCTTTATCCTGAACCGGAATGACCAGG AGGCCACGCTGGAAATGCTCTTTCCTAGCCGGACTACGTGGGTGTACTCC ATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCCCACCTGTATTC TCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACC CCATCGCTCACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTT TCCACCAAGATCCAGGACACCAAAGGCTGCCGGGCGTGCTGTGTGGCGGA GGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCCG TTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGG CAGGTGCTGTTCCCACTGCCGACGCCTCTGTCCGTGTTCGCGCTGCTGAC CGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGTGAGCCCCGGGC GGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCT TGCTGGCTGGGCGAGATGAGCACCGAGCACAGGGGACCCGTGCAGGTGAC CCAGGTAGAGGAAGATATGGTGATGGTGTTGATGGATGGCTCTGTGAAGC TGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGATC CCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCA GGCCTTCTGGAAGCATGGAGTGCAGGTGTGGGCTCTAGGCTCGGATCAGC TGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTCTGCTTGGCTCC CCCAGGCCTGTAGTGGTGGAGACACGCCCAGTGGATGATCCTACTGCTCC CAGCAACCTCTACATCCAGGAATGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYH
GSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYW
MAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPG
LNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSD
DDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEK
MKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRA
GNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVS
PGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGS
DQLLQELRDPTLTFRLLGSPRPVVVETRPVDDPTAPSNLYIQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 FLAG HPK1303.97 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU39742
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No