cDNA Clone USP27x

• Plasmid: pET28a-HALO_TEV-USP27x C87A
• Parent Plasmid: pET28a HALO TEV
• Expressed: HALO-USP27x C87A
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGTGTAAGGACTATGTATATGACAAAGACATTGAGCAAATTGCCAAAGAAGAGCAAGGAGAAGCTTTGAAATTACAAGCCTCCACCTCAACAGAGGTTTCTCACCAGCAGTGTTCAGTGCCAGGCCTTGGTGAGAAATTCCCAACCTGGGAAACAACCAAACCAGAATTAGAACTGCTGGGGCACAACCCGAGGAGAAGAAGAATCACCTCCAGCTTTACGATCGGTTTAAGAGGACTCATCAATCTTGGCAACACGTGCTTTATGAACTGCATTGTCCAGGCCCTCACCCACACGCCGATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGATGTGAGATGCCGAGTCCCGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTGTTTCGGGAGTTGTATTCTGGAAACCCGTCTCCTCATGTGCCCTATAAGTTACTGCACCTGGTGTGGATACATGCCCGCCATTTAGCAGGGTACAGGCAACAGGATGCCCACGAGTTCCTCATTGCAGCGTTAGATGTCCTGCACAGGCACTGCAAAGGTGATGATGTCGGGAAGGCGGCCAACAATCCCAACCACTGTAACTGCATCATAGACCAAATCTTCACAGGTGGCCTGCAGTCTGATGTCACCTGTCAAGCCTGCCATGGCGTCTCCACCACGATAGACCCATGCTGGGACATTAGTTTGGACTTGCCTGGCTCTTGCACCTCCTTCTGGCCCATGAGCCCAGGGAGGGAGAGCAGTGTGAACGGGGAAAGCCACATACCAGGAATCACCACCCTCACGGACTGCTTGCGGAGGTTTACGAGGCCAGAGCACTTAGGAAGCAGTGCCAAAATCAAATGTGGTAGTTGCCAAAGCTACCAGGAATCTACCAAACAGCTCACAATGAATAAATTACCTGTCGTTGCCTGTTTTCATTTCAAACGGTTTGAACATTCAGCGAAACAGAGGCGCAAGATCACTACATACATTTCCTTTCCTCTGGAGCTGGATATGACGCCGTTTATGGCCTCAAGTAAAGAGAGCAGAATGAATGGACAATTGCAGCTGCCAACCAATAGTGGAAACAACGAAAATAAGTATTCCTTGTTTGCTGTGGTTAATCACCAAGGAACCTTGGAGAGTGGCCACTATACCAGCTTCATCCGGCACCACAAGGACCAGTGGTTCAAGTGTGATGATGCCGTCATCACTAAGGCCAGTATTAAGGACGTACTGGACAGTGAAGGGTATTTACTGTTCTATCACAAACAGGTGCTAGAACATGAGTCAGAAAAAGTGAAAGAAATGAACACACAAGCCTACTGAGCGGCCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMCKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQDAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTTIDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU23252
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C87A (TGC to GCC) compared to ref sequence shown; Parental 23222