cDNA Clone TRIM21

• Synonyms: RO52, RNF81, SSA1
• Plasmid: pET156P Sumo Trim21
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Trim21
• Genbank: P19474
• Tag: SUMO
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGTCACATGCCCTATCTGCCTGGACCCCTTCGTGGAGCCTGTGAGCATCGAGTGTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGGGGGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCCAACATGGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAGTGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATGTGCCCAGTCTCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGCACAGGAGTACCAGGAGAAGCTCCAGGTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGAGAGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCAAAAAAACTTCCTGGTTGAAGAAGAACAGAGGCAGCTGCAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATCCTGGGGGAGAAAGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAGAGCTAGATCGAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTGATAATTGTCCTGGAAAGGAGTGAGTCCTGGAACCTGAAGGACCTGGATATTACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAGATGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAATCCGTGGCTGATACTTTCAGAAGATCGGAGACAAGTGAGGCTTGGAGACACCCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAGTTATCCTATGGTCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGATGTGACAGGAAAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCGCAGGAAGGGGCACTTTTTGCTTAGTTCCAAGAGTGGCTTCTGGACAATTTGGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCCCCTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGAGGCTGGCATGGTCTCCTTCTACAACATCACTGACCATGGCTCCCTCATCTACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGCCCTTCTTCAGTCCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCCACTGAATATTGGATCACAAGGATCCACTGACTATTGA

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVQMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGmasaarltmmweevtcpicldpfvepvsiecghsfcqecisqvgkgggsvcpvcrqrfllknlrpnrqlanmvnnlkeisqearegtqgercavhgerlhlfcekdgkalcwvcaqsrkhrdhamvpleeaaqeyqeklqvalgelrrkqelaekleveiaikradwkktvetqksrihaefvqqknflveeeqrqlqelekdereqlrilgekeaklaqqsqalqeliseldrrchssalellqeviivlerseswnlkdlditspelrsvchvpglkkmlrtcavhitldpdtanpwlilsedrrqvrlgdtqqsipgneerfdsypmvlgaqhfhsgkhywevdvtgkeawdlgvcrdsvrrkghfllssksgfwtiwlwnkqkyeagtypqtplhlqvppcqvgifldyeagmvsfynitdhgsliysfsecaftgplrpffspgfndggkntapltlcplnigsqgstdy*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22292
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Extra bases between GG of Sumo tag and Initiating Methionine of ORF deleted