cDNA Clone TRPM7

• Synonyms: CHAK, CHAK1, LTRPC7, TRP-PLIK
• Plasmid: pGEX6P-1-TRPM7 1158-end S1504P
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRPM7 1158-end S1504P
• Genbank: NM_017672.4
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCAAAACTTTTCTTAACAGAAGAAGATCAAAAGAAACTTCATGATTTTGAAGAGCAGTGTGTTGAAATGTATTTCAATGAAAAAGATGACAAATTTCATTCTGGGAGTGAAGAGAGAATTCGTGTCACTTTTGAAAGAGTGGAACAGATGTGCATTCAGATTAAAGAAGTTGGAGATCGTGTCAACTACATAAAAAGATCATTACAATCATTAGATTCTCAAATTGGCCATTTGCAAGATCTTTCAGCCCTGACGGTAGATACATTAAAAACACTCACTGCCCAGAAAGCGTCGGAAGCTAGCAAAGTTCATAATGAAATCACACGAGAACTGAGCATTTCCAAACACTTGGCTCAAAACCTTATTGATGATGGTCCTGTAAGACCTTCTGTATGGAAAAAGCATGGTGTTGTAAATACACTTAGCTCCTCTCTTCCTCAAGGTGATCTTGAAAGTAATAATCCTTTTCATTGTAATATTTTAATGAAAGATGACAAAGATCCCCAGTGTAATATATTTGGTCAAGACTTACCTGCAGTACCCCAGAGAAAAGAATTTAATTTTCCAGAGGCTGGTTCCTCTTCTGGTGCCTTATTCCCAAGTGCTGTTTCCCCTCCAGAACTGCGACAGAGACTACATGGGGTAGAACTCTTAAAAATATTTAATAAAAATCAAAAATTAGGCAGTTCATCTACTAGCATACCACATCTGTCATCCCCACCAACCAAATTTTTTGTTAGTACACCATCTCAGCCAAGTTGCAAAAGCCACTTGGAAACTGGAACCAAAGATCAAGAAACTGTTTGCTCTAAAGCTACAGAAGGAGATAATACAGAATTTGGAGCATTTGTAGGACACAGAGATAGCATGGATTTACAGAGGTTTAAAGAAACATCAAACAAGATAAAAATACTATCCAATAACAATACTTCTGAAAACACTTTGAAACGAGTGAGTTCTCTTGCTGGATTTATTGACTGTCACAGAACTTCCATTCCTGTTCATTCAAAACAAGCAGAAAAAATCAGTAGAAGGCCACCTACCGAAGACACTCATGAAGTAGATTCCAAAGCAGCTTTAATACCGGATTGGTTACAAGATAGACCATCAAACAGAGAAATGCCATCTGAAGAAGGAACATTAAATGGTCTCACTTCTCCATTTAAGCCAGCTATGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACTTGATGAGGTTATCACAGAGCATTCCATTTACACCTGTGCCTCCAAGAGGGGAGCCTGTCACAGTGTATCGTTTGGAAGAGAGTTCACCCAACATACTAAATAACAGCATGTCTTCTTGGTCACAACTAGGCCTCTGTGCCAAAATAGAGTTTTTAAGCAAAGAGGAGATGGGAGGAGGTTTACGAAGAGCTGTCAAAGTACAGTGTACCTGGTCAGAACATGATATCCTCAAGTCAGGGCATCTTTATATTATCAAATCTTTTCTTCCAGAGGTGGTTAATACATGGTCAAGTATTTATAAAGAAGATACAGTTCTGCATCTCTGTCTGAGAGAAATTCAACAACAGAGAGCAGCACAAAAGCTTACGTTTGCCTTTAATCAAATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTGAAGTTTTCCTGCTGTATTGCCATTCAGCAGGACAGTGGTTTGCTGTGGAAGAATGTATGACTGGAGAATTTAGAAAATACAACAATAATAATGGAGATGAGATTATTCCAACTAATACTCTGGAAGAGATCATGCTAGCCTTTAGCCACTGGACTTACGAATATACAAGAGGGGAGTTACTGGTACTTGATTTGCAAGGTGTTGGTGAAAATTTGACTGACCCATCTGTGATAAAAGCAGAAGAAAAGAGATCCTGTGATATGGTTTTTGGCCCAGCAAATCTAGGAGAAGATGCAATTAAAAACTTCAGAGCAAAACATCACTGTAATTCTTGCTGTAGAAAGCTTAAACTTCCAGATCTGAAGAGGAATGATTATACGCCTGATAAAATTATATTTCCTCAGGATGAGCCTTCAGATTTGAATCTTCAGCCTGGAAATTCCACCAAAGAATCAGAATCAACTAATTCTGTTCGTCTGATGTTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPKLFLTEEDQKKLHDFEE
QCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSL
QSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLA
QNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDP
QCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELL
KIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETV
CSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRV
SSLAGFIDCHRTSIPVHSKQAEKISRRPPTEDTHEVDSKAALIPDWLQDR
PSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPV
PPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRR
AVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREI
QQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTG
EFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENL
TDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLK
RNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML*AA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU41293
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S1504/P change. Also contains known variation T1482I and 2 other silent variations.