cDNA Clone C9orf84 (mouse)

• Synonyms: AI481877, Gm426
• Plasmid: pcDNA5-FRT/TO-GFP C9orf84m Bam Not
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-C9orf84m
• Genbank: XM_001476641.2
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCGTGATGGCATTGAACGGAAGAACTATGTTTCCAGCCTTCAAATATTATGCAATAGACTATTTACAAGAGGACATAATTAAAGAGAGGCTTTACAGAGATGCTTTATTGCTTCAGATTCCTTCATGTTTAAATCAAGATAAAAACATTATTGATGATAAATATAGGACTCCATGGACAAGAGCTATTCCAGTTCAGGAAATGGAAGATAATTCAGTCTTGGAGCAGTGGAGAACAAGATTCTGTGTGGAGGGTGTTCCTGAGAAAAAGACAGTTACAGGAGTGATGATTAATGGCACATTTGAGGAAATTGTTCCAAGTTCCAATCCAAACTCTCCACCTGGAATAGAGAACGATAAACTGTTCCCCAGTAAGGACTACGTAGACGACTTTATTCCTGTCAAGTGTTCCTTGTACTACCCCGGAGTGAAAGCGGAGCACCAAGGATTGCTTATTGATGAGGAAATGATTTTTATGAATAAAGCAATGGACAACCATCTACCAACTGTGAATGGTCTCCTGAGCAGACTGAAGCTCTATCTGGTGAAGGACCCATTTTTAGATTT-CAAAGAAGAGCTCTCTGGAAAGGATAATTTCACGGAGTATTTCTCTGTTCAAGAATGTTCGGAACCTTTTGTGAGAGATTTCCATATGGCTGAGGAGACCTTTTGTAAGAAGAAACTACCATCAGTTTTTCCTTCTGGGTTTAAATCCTTGATATCTACAAATCCCAAACAAGAAATTCTCATTCTACCACCATCAAAACTGAAGAAACCATTAAACTCGATACCGAAAATAATGGACTCTGTAGATGAAAGTGAATGTTTCAAAGGAGACATTACTTCCAAGCATGAATTTGATACTGAGGATATAAAATGCAACTCCACAGAAAACTTAACATTTGCAAGCCTTTGTGAACCAGAGTGCAGTGAGCCAGGAGACCTAGAAATGCCACCAACCCATCTAATCTTACCAAGGCAGCATTCTGCAGTG-AGTTCATTACACATGGGATTTCAGACATTTCCGTTTTCTGCTACATGTAAAATTAATTTGCTTAGTGCTGGAGAATCAGCTAATAAATATTGTATGCTGTGGCAACTGGGAGGCTGCAGAAACTCTTGGGTCTCGTTCTTGCTTACAGTTCCAAGATTTCAAGAACCCAGCAGTCAATATTCACTTGCAGATATGAGGAACATCTTTTCTGTCAAAGGAGACAGCCTTGTGATTAACCCTGCAAAAGCAAAGGGGTGGAGACAAGCACGACTACATCCAATAATGGCTGAAACGCTGGCGCATCTGAAGGCGTATTTGTGTCATAATGGTCTGTCTTCTCAGGAGACTAAGCTGGAGATCTTTCTGCCTACAAAAGTGTTTCAGCTGGAGTCCTGGCTAGAACTTAAGAGTCGTCCCTTGCCCATTGTGCCAATCAGTGAGAAATCTACAGATGTTCACCAGTTACATCCACAGAAGAGACCAATACCCTCTTCAGAAAAGGAAGTACCACACCTTTGTTTGTCCGGTGAAAGTATCTCTGTTAAAAAATCCAAAGTAGAAGCAAACCCAAAGAATGATCAAGAACCAGAAGCTAGAATAATGCAGAAGCCGGAGAACAGCTGTGTTGGACTTGGCTGTTCAAGTCAAGTGCCATCTGCTGAATCAGCTTCCTCTTCCCAGATCCAGGCATCTTATGATAAAAAACAAGATGATTTGGACCTTCTGAGCGAGTTTATTATTCTGAGAAGTAAACACCAGACTTTCCCTTCAGAAGCTGACGTCGATGTCAAAAGCCATGACCATGACCAAAACAATGAATTTCAAGATAAAGAAAAATATTCTTTGACTCTTCAAGAAGAAAGTCTTGTTGCTTCTAATAGTAAAGCTCCAGAGGAGAGATGTGAAGAAAGGACAGATGGCGTCATCGAGATCCCCGCTTCAGACACCCAGTGCCAAGCATACTGTCTCCTAGAAGCCACAGCGAATCCTATCTTAAAGGAGCTTGTTTGCCTCTGTACTTACCCTGCAGCCAATTGGAAATTCGCCACTGTCAATTTTGACCAAACAAGATTCTTCTTAAAGGAACAAGAAAAAAAGATAAATGATGCTACTCACCCCGATAAAAATGATGACAGAAAGATGACATTCAGGCATGCTGCTCTCTTACATCTCCTGATAACAATTAGAGACGTCCTTTTAACATGCAACTTGGATACTGCATTAGGATATTTGTCAAATGCAAAAGATATCTACAAAAGTGTGTTAGACTCCCGTTTGGATAACATTTGGAGACAGCTGAAAATTTTACAGTTTATTAAGGAAAAAAGGCCTAAAAGCAACTATAAGATCCAAGAATTGCAGTGTCAAATACTAAGTAGGCTACAGAATCAACAACAAATGAAGGTACTGATTATAATAAGAATGGACTCAGATGGAGAAAAGCATCTGCTTATTAAAACACTTAAGAAAATAGAAGGTTTAACAATGACTGTTTTACGTTCTAATGATAGAAAAAAAATTCTGGAAACTACCAGTATTTTAAAAGGTACGAATGCCTGCGTGGTTGTGCACAACCACAGTATCGGAGCGGATTTCCCCTGGAGCAGCTTCTCGCTCGTGGTGGAGTACAACCATGTGGGGCACTCTTGCTGGGCCGAGCACTGCCAACTGTTGGATATACCTTTTCTGGCCTTTAAAGTCGCTGTTCCAGACACAGCTCTGCAGAGAGATGCCTTGCTGGATGGATTTGGTGGGTTTCTCTTGAAAATTCCCATTCCATATGTATTTTTTGCATCTGAAGGACTTCTCAATACTCCAGAGATACTCCGGCTGCTGGAGTCCAACTATAATATCACACTAGTGGAGAGATGCTGCTGTGGGTCACTGAAACTCTTTGGAAG-TACAGAGTGCTATGT---GGTGGTGAC-AGTCGACGAGCATACTGCTATAGTTGTGCAGGACCTAGAAGAGTTACATCATGAGAAGGCATCAGACAATATCATCATGAGATTAATGGCACTGTCGTTCCAGTATAGCTGTTGCTGGATAATACTATATTCCAAGGAAACACTAAATTCACAATACCATCTTACAGAAGAGACACTTCGTCACCTCGCACAGGTTTATGCAGCTCTGGTTTCCTCGGGCCTGAAATCTGAAGAGCTGGATGTAAAGCTTATAATTGCCCCTGGAGTAGAAGAGACTGCTTTAATAATTCGACAAATTGCAGACCACAATTTAATGACGTCGACGAGAGATCCCCATGAATGGTTGGATAAATCCTGGGTGGAAGTTTCACCTTCTAAGGAGGAAATGAGCCTACTTGATTTTCCCTGTATAAACCCACTGGTGGCTCAGCTCATGTTACACAGGGCGCCTTCGCTGCACTGGCTCCTGATAGCAACGCCTGCTGAGCTCCAGGAACTCCTGCCCCAGGTTCCAGGCAAAGTACTGAAGCATTTTTGCAGCATCACTTCCTTGTTCAAGATTAGTTCTCCCTCCATGACAAAGTCTTCTCAGATTTCATCACTTCAGGAAGATATGAATCAAACTGATCTCTTTATTTCTCAGAGTTCAGCTCCTATCATTCAAGAACAGGAGGAATATTATCCCTATGAAGACTCGGAAGGCACAAGCTCTTCTCCTGTGGAGCTAAGAGCAACTCCATGCATGCTGCCATCTGCAGCACCGCACAGTCAGAGGGGCTGCTGGGAGGACCCCAGCTGTGGCCCTGACCCCGTGCAGAATAACCCTTCCCTAATGAATGCAGAATCAAAGAAAGTCACCTGGCCCTCCGTTCCCAGCTGGAGTGATTCAGAATCAGATGTCTTTTCTCTGGCCCGAACACAAGTAAGTTGTGAACCCATAATGACACTAACTGATTCTCAAAGGAGAGGCACAAATGGTTTTGTAAATTGTCCCGAAGCCAAAGGACCCAGAAACATGCAAGTGTCCACCCCTGTCTTTTTACCAGAGAACTCCCAGTCCCACCTTCACTGGGACTTTAAGAAAAACAGTTGTAGAAAACAGATTTACTCGTTCAATCCAAGCTGTGGAACAGAACAGACTACTTACAACAAATGGTACTCTTGGAAAGATGACTTCTCCAGTAATCAGCCCGAGTGTCTGTGGGATGAGATGGAAGATGTCACATACAGAAATGCAAATGCTGGGACCCGGGAGACTTTTTGGAGAGAACTGCCAGCTGTTCCAAGTTGGGATTCACCCTGTGCTTCTGATTCTAATGCAAATCAAAGAGGGTTCAAGGGCCTTGATTTCTGCCAAAGAGCTGGAAACTACTTAGGACAGAGAAGCCTCCCTGTGTCTTCATCTAATTGGGGAGATTACAAAACACCAACAGACTTAATGTACTCACAAGTACCACAACCCAAGAAACGACGTCTAATGTATGAGAAAGTCCCTGGAAGAGTGGATGGGCAAACCCGGCTGAAGTTTTTATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSVMALNGRTMFPAFKYYAIDYLQEDIIKERLYRDALLLQIPSCLNQDKNIIDDKYRTPWTRAIPVQEMEDNSVLEQWRTRFCVEGVPEKKTVTGVMINGTFEEIVPSSNPNSPPGIENDKLFPSKDYVDDFIPVKCSLYYPGVKAEHQGLLIDEEMIFMNKAMDNHLPTVNGLLSRLKLYLVKDPFLDFKEELSGKDNFTEYFSVQECSEPFVRDFHMAEETFCKKKLPSVFPSGFKSLISTNPKQEILILPPSKLKKPLNSIPKIMDSVDESECFKGDITSKHEFDTEDIKCNSTENLTFASLCEPECSEPGDLEMPPTHLILPRQHSAVSSLHMGFQTFPFSATCKINLLSAGESANKYCMLWQLGGCRNSWVSFLLTVPRFQEPSSQYSLADMRNIFSVKGDSLVINPAKAKGWRQARLHPIMAETLAHLKAYLCHNGLSSQETKLEIFLPTKVFQLESWLELKSRPLPIVPISEKSTDVHQLHPQKRPIPSSEKEVPHLCLSGESISVKKSKVEANPKNDQEPEARIMQKPENSCVGLGCSSQVPSAESASSSQIQASYDKKQDDLDLLSEFIILRSKHQTFPSEADVDVKSHDHDQNNEFQDKEKYSLTLQEESLVASNSKAPEERCEERTDGVIEIPASDTQCQAYCLLEATANPILKELVCLCTYPAANWKFATVNFDQTRFFLKEQEKKINDATHPDKNDDRKMTFRHAALLHLLITIRDVLLTCNLDTALGYLSNAKDIYKSVLDSRLDNIWRQLKILQFIKEKRPKSNYKIQELQCQILSRLQNQQQMKVLIIIRMDSDGEKHLLIKTLKKIEGLTMTVLRSNDRKKILETTSILKGTNACVVVHNHSIGADFPWSSFSLVVEYNHVGHSCWAEHCQLLDIPFLAFKVAVPDTALQRDALLDGFGGFLLKIPIPYVFFASEGLLNTPEILRLLESNYNITLVERCCCGSLKLFGSTECYVVVTVDEHTAIVVQDLEELHHEKASDNIIMRLMALSFQYSCCWIILYSKETLNSQYHLTEETLRHLAQVYAALVSSGLKSEELDVKLIIAPGVEETALIIRQIADHNLMTSTRDPHEWLDKSWVEVSPSKEEMSLLDFPCINPLVAQLMLHRAPSLHWLLIATPAELQELLPQVPGKVLKHFCSITSLFKISSPSMTKSSQISSLQEDMNQTDLFISQSSAPIIQEQEEYYPYEDSEGTSSSPVELRATPCMLPSAAPHSQRGCWEDPSCGPDPVQNNPSLMNAESKKVTWPSVPSWSDSESDVFSLARTQVSCEPIMTLTDSQRRGTNGFVNCPEAKGPRNMQVSTPVFLPENSQSHLHWDFKKNSCRKQIYSFNPSCGTEQTTYNKWYSWKDDFSSNQPECLWDEMEDVTYRNANAGTRETFWRELPAVPSWDSPCASDSNANQRGFKGLDFCQRAGNYLGQRSLPVSSSNWGDYKTPTDLMYSQVPQPKKRRLMYEKVPGRVDGQTRLKFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU41135
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent T>C 546 bp to KO Internal BamHI knocked out, may be excised B/N for shuttling