cDNA Clone FAM83D

• Synonyms: C20orf129, CHICA
• Plasmid: pCMV5-HA FAM83D
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-FAM83D
• Genbank: NM_030919.2
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGGCGCGGGCCTGTTTGATTCAAAGGTTGCCTATAAAGCGGGACTGCACGCCGGTTTTTGTCCGAGGGCTGTCGAGTCCGAGCGCCGCCATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGTGCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGTGGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAAGGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGCCCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCAGAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAGATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCACAGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTCAAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCACCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGCAATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATCCTTCCTATCAGTAAgtcgac

Amino Acid Sequence

MARACLIQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAI
LRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMD
VFTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLN
SSNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGKAERKPHDCESSTV
SEEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMS
VSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELYLSDSLRNLNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFS
RVNLLAVRDVALYPSYQ*TGMYPYDVPDYAGSMARACLIQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTELFSESRRLALEE
LVAGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYRGATRVETHFQPR
GAGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITGNIYYARSGTKII
GKVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTLGNLLRMRLARLS
STPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVITRIASSQTTIWSR
STTTQTDMDENILFPRGTQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELYLSDSLRNLNKERQFHFAGI
RSRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU41049
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: May be useful to grow at 30oC to prevent rearrangement particularly in higher copy vectors.