cDNA Clone HPK1

• Synonyms: mitogen-activated protein kinase kinase kinase kinase 1, MAP4K1
• Plasmid: pEGFPC4 TEV HPK1 D155A S454A
• Parent Plasmid: pEGFPC4
• Expressed: GFP-Precission-TEV HPK1 D155A S454A
• Genbank: NP_001036065.1
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGGAAAACCTGTATTTTCAGGGCatggacgtcgtggaccctga
cattttcaatagagacccccgggaccactatgacctgctacagcggctgg
gtggcggcacgtatggggaagtctttaaggctcgagacaaggtgtcaggg
gacctggtggcactgaagatggtgaagatggagcctgatgatgatgtctc
cacccttcagaaggaaatcctcatattgaaaacttgccggcacgccaaca
tcgtggcctaccatgggagttatctctggttgcagaaactctggatctgc
atggaattctgtggggctggttctctccaggacatctaccaagtgacagg
ctccctgtcagagctccagattagctatgtctgccgggaagtgctccagg
gactggcctatttgcactcacagaagaagatacacagggacatcaaggga
gctaacatcctcatcaatgatgctggggaggtcagattggctgcctttgg
catctcggcccagattggggctacactggccagacgcctctctttcattg
ggacaccctactggatggctccggaagtggcagctgtggccctgaaggga
ggatacaatgagctgtgtgacatctggtccctgggcatcacggccatcga
actggccgagctacagccaccgctctttgatgtgcaccctctcagagttc
tcttcctcatgaccaagagtggctaccagcctccccgactgaaggaaaaa
ggcaaatggtcggctgccttccacaacttcatcaaagtcactctgactaa
gagtcccaagaaacgacccagcgccaccaagatgctcagtcatcaactgg
tatcccagcctgggctgaatcgaggcctgatcctggatcttcttgacaaa
ctgaagaatcccgggaaaggaccctccattggggacattgaggatgagga
gcccgagctaccccctgctatccctcggcggatcagatccacccaccgct
ccagctctctggggatcccagatgcagactgctgtcggcggcacatggag
ttcaggaagctccgaggaatggagaccagacccccagccaacaccgctcg
cctacagcctcctcgagacctcaggagcagcagccccaggaagcaactgt
cagagtcgtctgacgatgactatgacgacgtggacatccccacccctgca
gaggacacacctcctccacttccccccaagcccaagttccgttctccatc
agacgagggtcctgggagcatgggggatgatgggcagctgagcccggggg
tgctggtccggtgtgccagtgggcccccaccaaacagcccccgtcctggg
cctcccccatccaccagcagcccccacctcaccgcccatgcagaaccctc
actctggaacccaccctcccgggagcttgacaagcccccacttctgcccc
ccaagaaggaaaagatgaagagaaagggatgtgcccttctcgtaaagttg
ttcaatggctgccccctccggatccacagcacggccgcctggacacatcc
ctccaccaaggaccagcacctgctcctgggggcagaggaaggcatcttta
tcctgaaccggaatgaccaggaggccacgctggaaatgctctttcctagc
cggactacgtgggtgtactccatcaacaacgttctcatgtctctctcagg
aaagaccccccacctgtattctcatagcatccttggcctgctggaacgga
aagagaccagagcaggaaaccccatcgctcacattagcccccaccgccta
ctggcaaggaagaacatggtttccaccaagatccaggacaccaaaggctg
ccgggcgtgctgtgtggcggagggtgcgagctctgggggcccgttcctgt
gcggtgcattggagacgtccgttgtcctgcttcagtggtaccagcccatg
aacaaattcctgcttgtccggcaggtgctgttcccactgccgacgcctct
gtccgtgttcgcgctgctgaccgggccaggctctgagctgcccgctgtgt
gcatcggcgtgagccccgggcggccggggaagtcggtgctcttccacacg
gtgcgctttggcgcgctctcttgctggctgggcgagatgagcaccgagca
cagggggcccgtgcaggtgacccaggtagaggaagatatggtgatggtgt
tgatggatggctctgtgaagctggtgaccccggaggggtccccagtccgg
ggacttcgcacacctgagatccccatgaccgaagcggtggaggccgtggc
tatggttggaggtcagcttcaggccttctggaagcatggagtgcaggtgt
gggctctaggctcggatcagctgctacaggagctgagagaccctaccctc
actttccgtctgcttggctcccccaggcctgtagtggtggagacacgccc
agtggatgatcctactgctcccagcaacctctacatccaggaatgagcgg
ccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPGSEFP
GVDSKAAAENLYFQGMDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKAR
DKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQ
KLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIH
RDIKGANILINDAGEVRLAAFGISAQIGATLARRLSFIGTPYWMAPEVAA
VALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPP
RLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLIL
DLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCC
RRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVD
IPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPN
SPRPGPPPSTSSPHLTAHAEPSLWNPPSRELDKPPLLPPKKEKMKRKGCA
LLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLE
MLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHI
SPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQ
WYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKS
VLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPE
GSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQEL
RDPTLTFRLLGSPRPVVVETRPVDDPTAPSNLYIQE*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU43600
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: AH[S]EPSL- please mutate the serine in brackets