cDNA Clone SLX4

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO GFP SLX4(BTBD12) S1066A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-SLX4 S1066A
• Genbank: NM_032444 c2454t (silent)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGAAACTGAGTGTGAATGAGGCTCAGCTAGGCTTCTACTTGGGTTCACTTTCTCATCTGTCTGCCTGTCCTGGGATTGACCCTCGCTCCTCTGAAGACCAGCCTGAAAGCCTTAAAACTGGTCAGATGATGGATGAGTCTGATGAGGACTTTAAAGAACTCTGCGCTAGCTTTTTCCAAAGGGTGAAAAAACATGGAATCAAGGAAGTGTCAGGAGAAAGGAAGACACAAAAGGCTGCCTCAAACGGCACTCAGATAAGAAGCAAATTGAAAAGGACCAAACAAACTGCTACCAAGACCAAAACCCTTCAAGGCCCTGCAGAGAAGAAACCTCCGTCTGGCAGCCAGGCCCCTAGGACTAAAAAGCAAAGGGTAACCAAATGGCAAGCAAGTGAACCGGCCCACTCTGTGAATGGGGAGGGGGGTGTGCTTGCCTCTGCTCCAGATCCACCTGTGCTCCGGGAAACAGCACAAAACACCCAGACGGGTAACCAGCAAGAACCATCGCCAAACCTTTCCAGAGAGAAAACCAGAGAGAATGTGCCCAACAGCGACTCCCAGCCTCCTCCTTCCTGTTTGACAACAGCAGTGCCAAGTCCCTCCAAACCCCGCACAGCACAATTGGTCCTACAGCGAATGCAGCAGTTCAAGAGAGCAGACCCCGAGCGTTTGAGACACGCTTCAGAAGAGTGCTCCCTCGAGGCTGCGCGGGAAGAAAATGTCCCAAAGGACCCTCAAGAGGAGATGATGGCGGGGAATGTGTATGGGCTTGGGCCCCCTGCCCCAGAGAGCGACGCTGCGGTGGCCTTGACCCTGCAGCAGGAGTTTGCACGGGTAGGAGCATCGGCACATGATGATAGCCTGGAGGAAAAGGGTTTGTTCTTCTGCCAGATTTGTCAAAAGAACCTCTCAGCCATGAACGTGACCCGAAGGGAACAGCATGTGAACAGGTGCTTGGATGAAGCTGAAAAGACACTAAGACCTTCTGTGCCTCAGATCCCTGAGTGCCCGATTTGTGGGAAACCGTTTCTTACCTTAAAGAGCAGAACCAGTCACTTGAAGCAGTGTGCTGTGAAGATGGAGGTTGGCCCCCAGCTCCTGCTTCAGGCTGTGCGGCTGCAGACAGCACAGCCTGAGGGTAGCAGCAGCCCACCCATGTTCAGCTTCAGTGATCACAGTAGAGGTCTGAAACGGAGAGGACCCACCAGCAAGAAGGAGCCACGGAAGAGGCGGAAGGTGGACGAGGCACCGTCCGAGGACCTGCTGGTGGCCATGGCTCTGTCCCGGTCGGAGATGGAGCCGGGTGCGGCTGTACCAGCGCTCAGGCTGGAAAGTGCCTTTTCTGAGAGGATAAGACCAGAAGCAGAGAATAAAAGTCGCAAGAAGAAACCCCCGGTATCCCCCCCATTGTTGTTAGTCCAGGACTCTGAAACCACAGGCCGACAGATAGAGGACCGTGTGGCCCTGCTCCTCTCTGAGGAAGTGGAATTGTCTAGCACGCCACCACTTCCTGCCAGCAGGATTTTAAAGGAAGGGTGGGAAAGAGCGGGCCAGTGTCCTCCTCCACCTGAACGCAAGCAGAGCTTTCTGTGGGAGGGCAGCGCACTGACTGGGGCCTGGGCCATGGAGGACTTCTACACGGCCAGGCTGGTCCCTCCTCTCGTGCCCCAGCGGCCTGCCCAGGGCCTTATGCAGGAGCCCGTGCCGCCTCTGGTGCCACCTGAGCACTCAGAGCTGAGCGAGCGAAGGTCACCCGCTCTCCACGGCACCCCCACTGCAGGCTGTGGCTCCAGGGGCCCGTCGCCTTCGGCCAGCCAGAGGGAGCACCAGGCCCTGCAGGACCTCGTGGACCTGGCGAGGGAGGGACTGAGCGCCAGCCCGTGGCCCGGCAGTGGGGGCCTGGCTGGCTCGGAAGGGACTGCAGGGTTGGACGTGGTGCCCGGCGGCCTTCCTCTGACTGGGTTTGTGGTGCCATCGCAGGACAAGCACCCGGACAGGGGCGGCCGAACCTTGCTCTCCCTCGGGCTGCTGGTTGCTGACTTTGGCGCCATGGTCAATAACCCACACCTGAGTGATGTCCAGTTTCAGACGGACAGCGGGGAGGTGCTTTACGCCCACAAGTTCGTGCTTTATGCCCGATGCCCGCTCCTCATCCAGTATGTGAACAATGAAGGCTTCTCCGCTGTAGAGGACGGGGTTCTGACCCAGCGTGTCCTGCTGGGTGACGTGAGCACCGAGGCCGCCCGCACGTTCCTGCACTATCTCTACACTGCGGACACTGGCCTTCCTCCTGGCCTTAGCTCTGAGCTGAGCTCCCTGGCCCACAGGTTTGGCGTGAGTGAGCTCGTTCACCTGTGCGAACAGGTGCCTATTGCCACTGACTCAGAGGGCAAACCATGGGAGGAGAAGGAAGCAGAGAATTGCGAAAGCAGGGCCGAGAATTTCCAGGAACTTTTGAGGTCAATGTGGGCAGATGAAGAGGAGGAAGCGGAGACTTTGTTGAAATCCAAGGACCACGAAGAAGATCAAGAAAACGTGAATGAAGCAGAAATGGAAGAAATTTATGAATTTGCAGCTACTCAGCGAAAGCTTCTCCAGGAAGAAAGGGCAGCGGGTGCCGGCGAGGACGCTGACTGGCTGGAGGGTGGCAGTCCGGTTTCTGGGCAACTCCTAGCAGGTGTCCAGGTGCAGAAACAGTGGGACAAGGTGGAGGAGATGGAGCCGTTGGAGCCAGGAAGAGATGAGGCCGCCACCACCTGGGAGAAGATGGGACAGTGCGCTCTCCCGCCACCCCAGGGCCAGCACTCAGGGGCACGGGGAGCAGAGGCCCCTGAGCAGGAGGCGCCAGAGGAGGCGCTTGGCCATTCCAGCTGCTCCAGCCCTTCCAGGGACTGCCAGGCAGAGAGAAAAGAAGGCTCTCTTCCGCACTCAGATGATGCCGGGGATTACGAACAGCTCTTCTCATCAACTCAGGGAGAGATCTCAGAGCCGTCCCAAATAACAAGTGAGCCCGAGGAACAAAGTGGCGCTGTCAGGGAAAGGGGGCTGGAGGTTTCTCATCGCCTGGCTCCCTGGCAGGCATCTCCACCGCACCCGTGCCGCTTCCTATTGGGGCCTCCCCAGGGCGGGAGTCCCCGCGGGTCTCATCACACAAGTGGGTCGTCCCTGTCAACACCCCGGTCCCGTGGCGGAACTGCCCAGGTGGGCTCCCCAACCTTGCTGTCTCCAGCTGTGCCATCAAAGCAGAAAAGGGACAGGAGCATCCTCACGCTGTCTAAAGAGCCAGGGCACCAGAAAGGCAAAGAGCGTCGGTCCGTGCTGGAGTGCAGAAATAAGGGGGTCCTGATGTTCCCAGAAAAATCTCTGTCTATTGACCTAACCCAGTCAAATCCTGACCATTCGAGCTCCAGATCTCAGAAATCTTCATCCAAACTGAACGAAGAAGATGAGGTCATCCTCTTACTGGACTCGGATGAGGAGCTGGAGCTAGAACAAACCAAAATGAAGTCCATTTCTAGTGATCCTCTGGAAGAAAAGAAAGCTCTAGAAATTAGCCCTAGGTCCTGTGAGCTGTTTTCCATCATTGATGTTGATGCAGATCAGGAACCTTCCCAGAGCCCACCAAGAAGCGAAGCTGTGCTGCAGCAGGAGGATGAGGGGGCGCTGCCGGAGAATCGGGGCTCTTTGGGCAGGAGAGGGGCTCCCTGGCTGTTCTGTGACCGTGAGAGCAGCCCCAGCGAGGCCAGCACCACAGACACCTCGTGGCTGGTGCCCGCCACCCCGCTGGCCAGCAGAAGCCGTGACTGTTCTTCCCAGACCCAAATCAGCAGCCTCAGGAGCGGGCTGGCCGTGCAGGCGGTGACTCAGCACACGCCCAGGGCCTCAGTAGGAAACAGGGAAGGGAACGAAGTCGCACAGAAGTTTTCTGTCATCAGGCCCCAGACACCACCGCCCCAGACACCGTCCTCATGCCTCACTCCCGTCTCTCCAGGAACTTCTGACGGCAGAAGGCAAGGCCACAGAAGCCCTTCCCGTCCCCACCCCGGGGGCCACCCGCACTCCTCTCCGCTGGCTCCACATCCCATCTCAGGGGACCGCGCCCACTTCAGCAGGCGGTTCCTGAAACACTCGCCGCCTGGGCCAAGCTTCCTGAACCAGACCCCAGCGGGTGAAGTGGTGGAAGTCGGAGACAGTGACGATGAGCAGGAGGTGGCCTCCCATCAGGCCAACAGAAGCCCCCCACTGGACAGTGACCCCCCAATTCCAATTGACGACTGCTGCTGGCACATGGAGCCCCTCTCGCCAATTCCCATTGACCACTGGAACCTGGAGCGGACCGGCCCCCTGAGCACCAGCAGCCCCAGCCGCAGGATGAACGAGGCCGCCGACAGCCGTGACTGTCGCTCCCCGGGACTCCTGGACACCACCCCCATCCGAGGAAGCTGCACTACCCAGAGGAAATTGCAAGAGAAGTCCTCGGGCGCGGGCTCCCTGGGGAACAGCAGGCCGAGCTTTCTGAATTCGGCTCTGTGGGACGTTTGGGACGGGGAAGAGCAGAGGCCTCCAGAGACCCCTCCTCCGGCCCAGATGCCAAGCGCTGGTGGAGCTCAGAAGCCCGAAGGGTTAGAGACACCCAAAGGTGCTAATCGGAAGAAGAACTTGCCCCCCAAAGTGCCCATAACGCCGATGCCACAGTATTCCATTATGGAGACGCCGGTGCTGAAGAAGGAACTGGATAGGTTTGGAGTCCGCCCTCTGCCTAAACGCCAGATGGTTCTGAAGCTGAAGGAGATATTCCAGTACACTCACCAGACCCTGGACTCAGACTCCGAGGACGAGAGCCAGTCCTCACAGCCGCTGTTGCAGGCGCCTCACTGCCAGACCCTCGCCTCCCAGACCTACAAGCCTTCAAGGGCAGGGGTCCATGCCCAGCAGGAGGCCACCACAGGACCTGGGGCCCATAGGCCCAAGGGACCTGCTAAGACCAAGGGCCCCCGACATCAAAGGAAGCATCATGAAAGCATCACACCCCCAAGCAGGTCGCCCACCAAGGAGGCACCTCCAGGCCTCAATGATGACGCCCAGATCCCAGCCTCTCAAGAATCCGTGGCCACCTCTGTGGATGGCAGTGACAGCTCCTTGAGCTCACAGAGTTCTTCCTCCTGTGAGTTTGGAGCGGCATTTGAGTCTGCAGGTGAAGAGGAGGGCGAGGGGGAGGTCAGTGCCTCGCAGGCAGCCGTGCAGGCGGCGGACACAGACGAGGCGCTGAGGTGCTACATCCGCTCCAAGCCGGCCCTGTACCAGAAGGTGCTGCTGTACCAGCCCTTTGAGCTGCGGGAGCTGCAGGCAGAGCTGAGGCAGAACGGCCTCCGTGTGTCCTCGCGCAGGCTGTTGGACTTCCTGGACACCCACTGTATCACCTTCACCACTGCCGCCACCCGCAGGGAGAAGCTCCAGGGCAGGAGGCGGCAGCCTCGGGGCAAGAAGAAGGTGGAGCGGAACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGFVVPSQDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSDVQFQTDSGEVLYAHKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVSTEAARTFLHYLYTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGKPWEEKEAENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHHTSGSSLSTPRSRGGTAQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45220
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.