cDNA Clone Miro2 (mouse)

• Plasmid: pet6HIS SUMO miro2 mouse 1-594
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO-Mouse Miro2 (1-594)
• Genbank: Q8JZN7
• Tag: 6His
• Tag: SUMO
• Species: Mouse

Sequence of Insert

agatctatgcggcgcgatgttcgcatcctgctgctgggcgaggcccaggt
ggggaagacgtctctgatcctgtcgctggtcggcgaggagtttcccgagg
aggtccctgcccgcgcggaggagataaccatccccgccgacgtcaccccg
gagaaggtgcccactcatatcgtggattactcagaagcggagcagacaga
ggaagaacttcaggaggagatccacaaggcaaatgtagtgtgtgtggtgt
acgatgtgtctgaggagaccaccattgagaagatccgaaccaagtggatc
ccgcttgtgaatggcaggacggcgacagggcccaggttgcccatcatcct
ggtgggcaataagtctgacctgcggccggggagtaccatggaggctgtgc
tgcccatcatgagccagtttcctgagatagagacctgcgtggagtgttca
gccaagcacttgaggaatatctcagagctgttctactatgcccagaaggc
tgttctgcatcccacagcccccctttatgaccctgaggccaaacagctca
ggcctgcatgcgcccaggcgctcacacgcatcttcaggctgtcggaccag
gaccgagaccacgggctgagcgacgaggaactcaatgccttccagaagtc
ctgctttggccatcccctggccccacaggccctggaggatgtgaagaggg
tggtgtgcaagaacgtgtcaggtggtgtgcagaatgaccggctgaccctg
gaaggcttcctcttcctgaacacactcttcatccagcgtggccggcacga
gaccacgtggaccatcctgcggcgctttggttacagtgactcactggagc
taacgcccgactacctctatccagcgctccatgtcccccctggctgcagc
acagagctcaaccatcgtggctaccagtttgtgcagcggatgttcgagaa
gcatgaccaggaccatgatggtgtcctctcgcccacggaactacagaatc
tcttcagtgtgttctcaggtgctccatggggccctgaactcttacacaca
gtccccactcaggctggctgtctgcctttgcatggctatctctgccagtg
gaccctcatgacctacttggatgtccagcaatgccttgcacaccttggct
acctgggttaccctaccctctgtgaacaggactctcaggcacaggctatc
acagttacccgcgagaagaagctggaccaggagaaagggcagacacagcg
cagcgttctcatgtgtaaggtgctgggagcccgaggagtgggcaagtcag
ccttcttgcaagccttccttggcaacagccttggggaagccagggacccc
cctgagaaattccccctgcataccatcaatacagtgcgggtcaacgggca
ggagaagtacctgattctgtgtgaagtgaatgctgacagcctgctggaca
cctctctagacactacctgtgatgtcgcctgcttaatgtttgacagcagt
gatcccaagacctttgtacactgtgctaccatatacaagcgttattacat
ggatgggcagaccccctgcctctttattgcctccaaagctgatctccctg
aaggtgttgcccccccagggctgtcacccgctgagttctgccggaggcac
cggctgcctgccccagcttcattctcctgcttgggacccgcaatgcccag
cacagatgtctttacccaacttgctaccatggccactttcccacacctgg
tgcacacagagctgcaccccacctccttctggctccgatgagtgctggtg
gctgtcggcacggctgtggctgctgtccttagcttctcactatacagggt
cctagtgaagagccgatgacgCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPARAEEITIPADV
TPEKVPTHIVDYSEAEQTEEELQEEIHKANVVCVVYDVSEETTIEKIRTK
WIPLVNGRTATGPRLPIILVGNKSDLRPGSTMEAVLPIMSQFPEIETCVE
CSAKHLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLS
DQDRDHGLSDEELNAFQKSCFGHPLAPQALEDVKRVVCKNVSGGVQNDRL
TLEGFLFLNTLFIQRGRHETTWTILRRFGYSDSLELTPDYLYPALHVPPG
CSTELNHRGYQFVQRMFEKHDQDHDGVLSPTELQNLFSVFSGAPWGPELL
HTVPTQAGCLPLHGYLCQWTLMTYLDVQQCLAHLGYLGYPTLCEQDSQAQ
AITVTREKKLDQEKGQTQRSVLMCKVLGARGVGKSAFLQAFLGNSLGEAR
DPPEKFPLHTINTVRVNGQEKYLILCEVNADSLLDTSLDTTCDVACLMFD
SSDPKTFVHCATIYKRYYMDGQTPCLFIASKADLPEGVAPPGLSPAEFCR
RHRLPAPASFSCLGPAMPSTDVFTQLATMATFPHLVHTELHPTSFWLR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43602
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee