cDNA Clone Synaptotagmin 1 (SYT1) Rat

• Plasmid: pcDNA3-pHluorin rat SYTI K326A, T328A, K331A
• Parent Plasmid: pcDNA3-pHluorin
• Expressed: pHluorin rat SYTI K326A, T328A, K331A
• Genbank: P21707
• Species: Human

Sequence of Insert

atggtgagtgccagtcatcctgaggccctggccgcccctgtcaccactgttgcgacccttgtcccacacaatgccactgagccagccagtcctggggaagggaaggaagatgccttttccaagctgaagcagaagtttatgaatgagctgcataaaattccattgccaccgtgggccttaatagccatagccatagttgcggtccttttagtcgtaacctgctgcttttgtgtctgtaagaaatgtttgttcaaaaagaaaaacaagaagaaggggaaggaaaagggaggaaagaacgccattaacatgaaagacgtgaaagacttagggaagaccatgaaggatcaggcccttaaggatgacgatgctgaaaccggactgactgatggagaagaaaaggaagagcccaaggaagaggagaaactgggaaagctccaatattcactggactatgacttccagaataaccagctgttggtgggaatcatccaggctgctgaactgcccgccctggacatggggggtacatccgatccatacgtcaaagtcttcctgctgcctgacaaaaagaagaaatttgagactaaagtccaccggaaaaccctcaatccagtcttcaatgaacaatttactTTCAAGGTACCCTACTCGGAATTAGGTGGCAAAACCCTGGTGATGGCTGTGTATGACTTTGATCGCTTCTCCAAGCACGACATCATCGGAGAGTTCAAAGTTCCTATGAACACCGTGGATTTTGGCCATGTGACCGAGGAGTGGCGCGATCTCCAGAGCGCTGAGAAAGAAGAGCAAGAGAAACTGGGTGACATCTGCTTCTCCCTCCGCTACGTCCCTACTGCCGGCAAACTGACTGTTGTCATTCTGGAAGCCAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCTGATCCCTACGTGAAGATTCACCTGATGCAGAACGGTAAGAGGCTGAAGAAGgcAAAGgCGACGATTgcGAAGAACACACTCAACCCCTACTACAACGAGTCCTTCAGCTTTGAAGTTCCGTTCGAGCAAATCCAGAAAGTGCAAGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGACGCGATCGGCAAAGTCTTCGTTGGTTACAACAGCACTGGGGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCCGGCGACCCATCGCACAGTGGCACACTCTGCAGGTAGAGGAGGAGGTTgatgccatgctggctgtcaagaagtaa

Amino Acid Sequence

MKILVAVAVFFLVSTQLFAEEIGANKLSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKTGDYDIPTTLENLYFQGELKTVDADMVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKAKATIAKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42421
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: K326A+T328A+K331A