cDNA Clone HELLS

• Synonyms: Helicase, lymphoid specific
• Plasmid: pCMV-FLAG-HELLS (mouse)
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG-HELLS
• Genbank: NP_032260.2 NM_008234.3
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GTCGACatggccgaacaaacggagcctgcggtgattactccagccatgctggaggaggaggaacagctcgaggctgcgggacttgagaaagagcggaagatgttagaagaggctcaaaagtcttgggacagagagtccactgaaattcggtaccgcagacttcagcatttgcttgaaaaaagcaatatctactcaaaatttttgttgactaaaatggaacaacagcaattggaggaacagaagaagaaagagaagttggagaaaaaaaaacggtcattaaaacttacagagggtaaaagtttGgttgatgggaatggagagaaaccagtcatgaaaaagaaaagaggaagagaagatgaatcttataatatttcagaggtcatgtcaaaagaggaaattttgtccgtagctaaaaaacataaggataatgaggatgaaagctcttccactacgagtctttgtgtagaagatattcagaaaaataaggattcaaatagtatgattaaagatagattgtctcaaactgttaggcagaattctaaattcttttttgacccagttcggaaatgtaacggacagcctgtaccctttcaacaaccaaagcatttcacaggaggagtaatgaggtggtaccaagtagaaggcatggaatggcttaggatgctttgggaaaatggaattaatggcattttagcagatgaaatgggtttgggaaagactgttcagtgcattgctacaattgcattgatgattcagaggggagtacctggaccttttcttgtttgtggccctttgtctacacttcctaactggatggctgaatttaaaagatttactccagaaattcctactctgctgtatcatggaacccgggaggaccgtcggaaattagtaaagaatatccacaaaagacaagggacactgcagattcatcctgtggtggtcacatcattcgagatcgctatgcgagaccagaatgctttacagcattgctattggaaatacttaatagtagatgaaggacacaggattaagaatatgaagtgccggctaatcagggagttaaaacggttcaatgctgataacaaacttcttttgactggtactcccttgcaaaacaatttatcagaactttggtcattgctaaactttttgttgccagatgtatttgatgacttgaaaagctttgagtcttggtttgaCatcactagtctttctgaaactgctgaagatattatagctaaagagagagaacagaatgttttacatatgctgcaccagattttaacgcctttcctactgagaagactgaagtctgatgttgctcttgaagtcccccctaagcgagaagtagttgtttatgcaccactttgcaataaacaagagatcttctatacagctattgtgaaccgcacaattgcaaacatgtttggatcctgtgagaaggaaacagttgagctaagtcctactggaagaccaaaacgacgaagtagaaagtcaataaattacagcgaactagatcagttccctagtgaattagaaaaactaataagtcaaatacagccagaagtaaacagagagaggactgttgtggaaggtaatatccctatagaatctgaagttaatctgaagctgcggaatataatgatgctacttcgaaagtgctgtaatcacccatatatgattgaatatcctattgaccctgtcacacaagaatttaagattgatgaagagttggtaacaaattctgggaagttcttaattttggatcgaatgctgcccgaacttaaaaaaagaggtcacaaggttctggtgttttcacaaatgacaagcatgttggacatcttgatggattattgccatcttagaaacttcatctttagcagacttgatgggtccatgtcttattcggaaagagaaaaaaatatatacagtttcaacacggatccagatgtttttcttttcttagtgagtacacgagcAggtggtttaggcataaacttgactgcagcagatacagtgatcatttatgacagtgattggaatcctcagtctgatctccaggctcaagatagatgtcatagaattggtcagacaaagccagttgttgtgtatcgtcttgtaacagcaaatactattgaccagaaaattgtggaaagagcagctgctaaaagaaagttggagaagttgataatccataaaaatcatttcaaaggtggtcagtctgggttaagtcaatctaagaatttcttagatgcaaaggaattaatggagttattaaaatctagagattatgaaagggaagtaaaaggatccagagagaaggtcattagtgacgaagatctagaattactgttggatcgaagtgatctcattgatcaaatgaaggcttcaagaccaattaaagggaagacagggatattcaaaatactagaaaattctgaagattccagtgctgaatgtttattttaaGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDMAEQTEPAVITPAMLEEEEQLEAAGLEKERKMLEEAQKSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLEKKKRSLKLTEGKSLVDGNGEKPVMKKKRGREDESYNISEVMSKEEILSVAKKHKDNEDESSSTTSLCVEDIQKNKDSNSMIKDRLSQTVRQNSKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPEIPTLLYHGTREDRRKLVKNIHKRQGTLQIHPVVVTSFEIAMRDQNALQHCYWKYLIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLCNKQEIFYTAIVNRTIANMFGSCEKETVELSPTGRPKRRSRKSINYSELDQFPSELEKLISQIQPEVNRERTVVEGNIPIESEVNLKLRNIMMLLRKCCNHPYMIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLVFSQMTSMLDILMDYCHLRNFIFSRLDGSMSYSEREKNIYSFNTDPDVFLFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLSQSKNFLDAKELMELLKSRDYEREVKGSREKVISDEDLELLLDRSDLIDQMKASRPIKGKTGIFKILENSEDSSAECLF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU23666
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: compared to NCBI ref clone has silent mutations at L99, D399, A659