cDNA Clone LRRFIP2

• Synonyms: leucine rich repeat (in FLII) interacting protein 2, HUFI-2
• Plasmid: pcDNA5-FRT/TO-FLAG-LRRFIP2 isoform 3 D158G
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: FLAG-LRRFIP2 isoform 3 D158G
• Genbank: NM_001134369.1, Gene ID 9209
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGGGACTCCTGCTTCTGGAAGGAAAAGAACACCTGTGAAAGACCGATTTTCTGCAGAAGATGAAGCTTTGAGTAACATTGCCAGAGAGGCAGAGGCAAGGCTGGCAGCAAAACGGGCTGCCCGGGCAGAAGCAAGAGATATACGCATGAGAGAACTGGAACGACAACAAAAAGAGTTGGATGAAAAATCTGACAAACAGTATGCTGAAAATTATACAAGACCTTCATCTCGAAATTCTGCCTCAGCAACAACCCCTCTAAGTGGAAACTCATCCAGACGAGGAAGTGGGGACACCAGCAGCTTAATAGATCCAGACACTTCATTAAGTGAATTGCGGGATATCTATGACCTTAAGGACCAGATACAGGATGTAGAAGGGAGATACATGCAGGGGCTTAAAGAACTAAAGGAATCTTTGTCTGAAGTGGAAGAAAAATACAAGAAAGCCATGGTTTCCAATGCACAGTTAGGCAATGAGAAGAACAATTTGATCTACCAAGTAGACACACTCAAGGATGTTATTGAAGAGCAGGAGGAACAGATGGCAGAATTTTATAGAGAAAATGAAGAAAAATCAAAGGAGTTAGAAAGGCAGAAACATATGTGTAGTGTGCTGCAGCATAAGATGGAAGAACTTAAAGAAGGCCTGCGGCAAAGAGATGAGCTTATTGAGAAACATGGCTTAGTTATAATCCCCGATGGCACTCCCAATGGTGATGTCAGTCATGAACCAGTGGCTGGAGCCATCACTGTTGTGTCTCAGGAAGCTGCTCAGGTCTTGGAGTCAGCAGGAGAAGGGCCATTAGATGTAAGGCTACGAAAACTTGCTGGAGAGAAGGAAGAACTACTGTCACAGATTAGAAAACTGAAGCTTCAGTTAGAGGAGGAACGACAGAAATGCTCCAGGAATGATGGCACAGTGGGTGACCTGGCAGGACTGCAGAATGGCTCAGACTTGCAGTTCATCGAAATGCAGAGAGATGCCAATAGACAAATTAGCGAATACAAATTTAAGCTTTCAAAAGCAGAACAGGATATAACTACCTTGGAGCAAAGTATTAGCCGGCTTGAGGGACAGGTTCTGAGATATAAAACTGCTGCTGAGAATGCTGAGAAAGTTGAAGATGAATTGAAAGCAGAAAAACGGAAGCTACAACGAGAGTTACGAACAGCACTGGACAAGATTGAGGAGATGGAGATGACCAACAGCCACCTGGCCAAGCGGCTGGAGAAGATGAAGGCCAATAGGACAGCACTTCTGGCCCAGCAGTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMGTPASGRKRTPVKDRFSAEDEALSNIAREAEARLAAKRAARAEARDIRMRELERQQKELDEKSDKQYAENYTRPSSRNSASATTPLSGNSSRRGSGDTSSLIDPDTSLSELRDIYDLKDQIQDVEGRYMQGLKELKESLSEVEEKYKKAMVSNAQLGNEKNNLIYQVDTLKDVIEEQEEQMAEFYRENEEKSKELERQKHMCSVLQHKMEELKEGLRQRDELIEKHGLVIIPDGTPNGDVSHEPVAGAITVVSQEAAQVLESAGEGPLDVRLRKLAGEKEELLSQIRKLKLQLEEERQKCSRNDGTVGDLAGLQNGSDLQFIEMQRDANRQISEYKFKLSKAEQDITTLEQSISRLEGQVLRYKTAAENAEKVEDELKAEKRKLQRELRTALDKIEEMEMTNSHLAKRLEKMKANRTALLAQQ*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU23602
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 3, D158G mutation plus silent mutation at Ala155,