cDNA Clone Miro1

• Synonyms: ARHT1, FLJ11040, FLJ12633, MIRO-1, MIRO1, hMiro-1, mitochondrial Rho (MIRO) GTPase 1, mitochondrial Rho 1, mitochondrial Rho GTPase 1, rac-GTP binding protein-like protein, rac-GTP-binding protein-like protein, ras homolog gene family, member T1
• Plasmid: pet6HIS SUMO Miro1
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6-His-SUMO- Miro1 (full)
• Genbank: Q8IXI2
• Tag: SUMO
• Species: Human

Sequence of Insert

GGAATGAAGAAAGACGTGCGAATTCTGCTGGTGGGAGAACCTAGAGTTGGGAAGACATCACTGATTATGTCTCTGGTCAGTGAAGAATTTCCAGAAGAGGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGTCACCCCAGAGAGAGTTCCAACACACATTGTAGATTACTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGTATAGTGTATGCCGTTAACAACAAGCATTCTATTGATAAGGTAACAAGTCGATGGATTCCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTTTAATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGTATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAAAACCTGAAGAACATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAAGCCCTTACTCGTATATTTAAAATATCTGATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACTCCATTAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTTACACACACTTTTTATCCAGAGAGGGAGACACGAAACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTGTTCCCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCTTTGACAAGCATGATTTGGATAGAGACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAGAGGCTGGATAACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATATTTGGGCTATCTAGGCTATTCAATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGATGTAATGTAATTGGAGTGAAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATTCGTGAAGATCATAAATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATCATTTGTGATGTTGTATGCCTGGTATATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGCAGAATACCTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACCACAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCGCACGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTATTGAAACAGCGATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43066
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: tv3. in pET15-6xHis-SUMO vector