cDNA Clone UbcH7

• Synonyms: E2-F1, L-UBC, UBE2L3, UbcM4
• Plasmid: pET156P UBCH7 C86K P88D H119D
• Expressed: 6His UBCH7 C86K P88D H119D
• Genbank: AAH53368
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAAATCCGCAAATGTGGGATGAAAAACTTCCGTAACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGATAAGGGAGCCTTCAGAATCGAAATCAACTTTCCAGCAGAGTACCCATTCAAACCACCGAAGATCACATTTAAAACAAAGATCTATCACCCAAACATCGACGAAAAGGGGCAGGTCAAGCTGGATGTAATTAGTGCCGAAAACTGGAAGCCAGCAACCAAAACCGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCCTGAGGACCCGCTTCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCTGAGGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGACTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVKLDVISAENWKPATKTDQVIQSLIALVNDPQPEDPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23539
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C86K oligos only to be used in P88D background. Silent mutation a423g (of UbcH7 ref)