cDNA Clone UBQLN3

• Plasmid: pGEX6P-1-UBQLN3 aa364-497
• Parent Plasmid: pGEX6P1
• Expressed: GST-UBQLN3 aa364-497
• Genbank: NM_198623.2
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCcagggtactgtgccaacttcaaatccaagccaggaatcaccactttcaggaaacagagttcctccaactttaccatcatcccctaaatctgggtcaggccaatctctgcccaaagagtcagtagcaatcaagggaaagtcatcatgcccagctttcttgagacactcaactgagaacagtactggacaaggtggaagcctacatgatgcagggaagggctctactggccccagtactagcttgcccaatcttacctctcagataggagattctgccaacagatcctcgtttgtttctacaccatcttcccttatgtcagctactcctggagtcccagaatctccctggcttcctccaacaggttactcaagatctctgagatcagcgggcacaaatcaagtctgaGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQGTVPTSNPSQESPLSGNRVPPTLPSSPKSGSGQSLPKESVAIKGKSSCPAFLRHSTENSTGQGGSLHDAGKGSTGPSTSLPNLTSQIGDSANRSSFVSTPSSLMSATPGVPESPWLPPTGYSRSLRSAGTNQV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23464
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone harbours silent mutations at Phe415 (c1245t) and Thr424 (t1272c)