cDNA Clone SKP1 (yeast)

• Synonyms: YDR328C, MGO1
• Plasmid: pRS413 SKP1p-3xHA-SKP1 S162D -SKP1 3’UTR
• Parent Plasmid: pRS413
• Expressed: 3xHA-SKP1 S162D
• Genbank: NM_001180636.1
• Tag: HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

GAGCTCGGTGGAGCGATCCGGAGGACAAAGCGGATGATGCAATGGTAATGGCAGCAAAAAAGTCAAAGAAAGAAGGCCAGGAAGTGTATATTAATGAGCAAGCTTTCCTATCACTATCATGGTGGATTCTTAACAAGGGGTGGTTGAGTTACAATGAAATAATTACAAATCAAATCGAAATTGAATTTGACGGCATACACCCAAGAGATACTCTAACGTTAGAAGAATTTAGTTCTCGTCTCACGAACATATTCCGGAACACGAATTCCCAAATATTCCAGCAAAATAATAATAACCTCACGTCTATTTTGCTTCCTAAAGATTCCAGTGGACAGGAGTTTCTTCTGTCACAGACGCTTGACGCGGATGCTTTAACAAGTTTTCACTCTAACACACTAGTTTTTAATCAATTGGTGAATGAACTGACCCAATGTATCGAAAGCACAGCAACATCCATAGTGTTAGAGTCTCTAATAAATGAATCATTCCATTTCATAATGAACAAGGTCGGCATAAAGACAATAGCCAAGAAAAAACCTGGCCAAGAAGATCAGCAACAGTATCAAATGGCAGTTTTCGCTATGTCGATGAAAGACTGTTGCCAAGAAATGCTTCAAACAACTGCCGGGTCTTCCCATAGCGGCAGCGTGAACGAATACCTGGCCACTCTGGATTCTGTGCAGCCGCTCGATGATCTGAGCGCCAGCGTATACAGCAACTTTGGCGTCTCCAGCTCGTTTTCCTTCAAGCCTTAATCTCTGAATAAGTACTGACACTCACACCAGAATATATATATATATAGCGTAATCTTTAATACAGTAAGTGTGCAATAGGTAACATGACATTACCCTGCAGCGATTTAACGGGCGTCTTGGAGAAAAGAAGAGAAAGGGAAGAAATAGGTTGATATATTGAGGGCAGAGAAAGTGATAGCCCAACGTTGGCAAAGATATAACAGAAAAGACGTTATAAACAATACAGGAATACTAACAAACGTAGCGCAGATCTAACCGGATCCAGTGAACCGTCAGAATTGGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGTACCCATACGATGTGCCAGATTACGCCATGTACCCATACGATGTGCCAGATTACGCCGTCGACATGGTGACTTCTAATGTTGTCCTAGTGAGTGGTGAGGGTGAACGATTCACCGTAGACAAGAAAATCGCGGAGAGATCCCTGCTATTGAAAAACTATCTGAACGATATGCATGACAGCAACCTACAAAACAACTCAGATTCAGAATCAGATTCAGACTCGGAAACGAACCACAAAAGCAAAGACAACAATAACGGTGACGACGACGATGAGGATGACGACGAGATTGTGATGCCCGTGCCCAATGTTCGGTCTTCCGTTTTGCAGAAGGTTATCGAATGGGCCGAGCACCACAGAGACTCTAACTTCCCTGACGAAGATGACGACGATTCCAGAAAGAGTGCTCCCGTGGACTCATGGGACAGAGAATTCCTGAAAGTGGACCAAGAGATGCTATACGAAATTATTCTGGCGGCAAACTACCTCAACATCAAGCCCTTGCTTGACGCTGGCTGCAAGGTGGTTGCTGAAATGATCAGGGGAAGGTCTCCCGAGGAAATTAGAAGAACGTTTAATATCGTCAACGACTTCACTCCAGAGGAGGAGGCTGCCATTAGGCGTGAAAATGAATGGGCTGAAGACCGTTAGCTCGAGCCTACATTTCCTCTGATGACTGTGATCCTTTCTTCCGATGATAATGCGCCATTTCAGAGCAAGATAGGCAAAAATAAGCAAACAAGGCTTGCTGTCATGCAATGTGTACGTTAGTCCACCATAACCAAGCATGTATGAATTATATATGTATACAGTTTACATAAACTTCAAACTACAATAAGATGTGTGGTCCATCGTCGTGTGTAGCGATAGGCGTCTTCTAAAAGCCGCAAAAGTGACAAGAAGAAAAAAGGAAAATTATTGAGTTATAAAAGCTATTGTTTACATGATGAGACGGGATAAATTTAGGACAGATAGCTGTAAACACTCCGTTTTGAATCATATTTGTGAAATATTTGGTCTAAATTTTAAGTCAAGTTTATTTTCTTCGAGCATTTCAACACCAGCTTAACCCTTTGCTTTAAATTATTCATCTAAAATAGGGTATATTGATTGCTAATCACATATAAATATATAGAAGCAGCAGCTGTACAGATATCGGGCCC

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAVDMVTSNVVLVSGEGERFTVDKKIAERSLLLKNYLNDMHDSNLQNNSDSESDSDSETNHKSKDNNNGDDDDEDDDEIVMPVPNVRSSVLQKVIEWAEHHRDSNFPDEDDDDSRKSAPVDSWDREFLKVDQEMLYEIILAANYLNIKPLLDAGCKVVAEMIRGRSPEEIRRTFNIVNDFTPEEEAAIRRENEWAEDR*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU23441
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone harbours TCT to GAT (S162D) compared to ref shown. insert sequence shows everything from promoter through to the 3' UTR (SacI-ApaI)