cDNA Clone Cyclin A

• Plasmid: pGEX6P1 Cyclin A (171-432) + CDK2
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C Cyclin A (171-432)
• Genbank: cyclin A NM_001237 CDK2 NM_001798
• Tag: GST
• Species: Human

Sequence of Insert

ggatccagtgttaatgaagtaccagactaccatgaggatattcacacata
ccttagggaaatggaggttaaatgtaaacctaaagtgggttacatgaaga
aacagccagacatcactaacagtatgagagctatcctcgtggactggtta
gttgaagtaggagaagaatataaactacagaatgagaccctgcatttggc
tgtgaactacattgataggttcctgtcttccatgtcagtgctgagaggaa
aacttcagcttgtgggcactgctgctatgctgttagcctcaaagtttgaa
gaaatataccccccagaagtagcagagtttgtgtacattacagatgatac
ctacaccaagaaacaagttctgagaatggagcatctagttttgaaagtcc
ttacttttgacttagctgctccaacagtaaatcagtttcttacccaatac
tttctgcatcagcagcctgcaaactgcaaagttgaaagtttagcaatgtt
tttgggagaattaagtttgatagatgctgacccatacctcaagtatttgc
catcagttattgctggagctgcctttcatttagcactctacacagtcacg
ggacaaagctggcctgaatcattaatacgaaagactggatataccctgga
aagtcttaagccttgtctcatggaccttcaccagacctacctcaaagcac
cacagcatgcacaacagtcaataagagaaaagtacaaaaattcaaagtat
catggtgtttctctcctcaacccaccagagacactaaatctgtaagaatt
caataattttgtttaactttaagaaggagatatacatatggagaacttcc
aaaaggtggaaaagatcggagagggcacgtacggagttgtgtacaaagcc
agaaacaagttgacgggagaggtggtggcgcttaagaaaatccgcctgga
cactgagactgagggtgtgcccagtactgccatccgagagatctctctgc
ttaaggagcttaaccatcctaatattgtcaagctgctggatgtcattcac
acagaaaataaactctacctggtttttgaatttctgcaccaagatctcaa
gaaattcatggatgcctctgctctcactggcattcctcttcccctcatca
agagctatctgttccagctgctccagggcctagctttctgccattctcat
cgggtcctccaccgagaccttaaacctcagaatctgcttattaacacaga
gggggccatcaagctagcagactttggactagccagagcttttggagtcc
ctgttcgtacttacacccatgaggtggtgaccctgtggtaccgagctcct
gaaatcctcctgggctgcaaatattattccacagctgtggacatctggag
cctgggctgcatctttgctgagatggtgactcgccgggccctattccctg
gagattctgagattgaccagctcttccggatctttcggactctggggacc
ccagatgaggtggtgtggccaggagttacttctatgcctgattacaagcc
aagtttccccaagtgggcccggcaagattttagtaaagttgtacctcccc
tggatgaagatggacggagcttgttatcgcaaatgctgcactacgaccct
aacaagcggatttcggccaaggcagccctggctcaccctttcttccagga
tgtgaccaagccagtaccccatcttcgactctgagcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 Cyclin A (171-432) + CDK259.52 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU43557
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No