cDNA Clone KIAA2022 (Mouse)

• Synonyms: C77370; A230051P11; C77386; Kiaa2022
• Plasmid: pBabe.puro.GFP.DU mouse KIAA2022
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-mouse KIAA2022
• Genbank: NM_001077354.2 GI:148539921 with silent changes g1923a, t2178g, a2179c and g2181c
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCATGGATAACCAACAAGATAAAGTTATTGCTGCCTCAGCCAATGGAGACAACAATCTGATTAATGGGGTCAAAAACAATGACTCAGAGGACCAGGAAGTGGCAATGAAGTCATTCGTAGCTCTAGAAGCTACTACTCCAATCCAGCCTATACCAGTGATACAAAAAGAGTCCCCGATGTTTCCCAGGGGCCTTCTTCCTCCGCCATCTAAGAAGCCGTGTATGCAGAGCCCTCCATCTCCTTTGGCCCTGATTGAAGCACCCGATCATTCTGCTAATAGTGCTTCTGTGAATGCCATCTCCCTCACATCTGGAGTTGCAAAAGGCCTGAACACATGGTCACTGCCCAACGAGTGTGAGAAAGCTCCATTTGCCATAATGGAACCTGCAGGCATGTCAGCTCTGAACGGAGACTGCCTCATGCAGCCAAGCAGGACTTGCTTAGGTTGCTTCATGGAATCAAAAGAAGCAGTAGATCCTGAACCAGGGATCAGTCTGAAAGTCAGTGATCTAAATCGAGATTATGAAACATGTGCAGTCTCTGACATAGGGATTCAATGCATTAATGCTGGGGAAAACATTAAGTATGGAGAGCAGCTGCTTTCAGACCAGCTGTTAGGCTTCCCCCTGCATAAGTCAAGGGCAGGAGATAGACGAGAATCTGAGAAACCTGACATCGACTTGGAAGATCCAACTCAGAAAAGTTATTATGAGGCATTACTATTAGATAAGTGCAACACGGAAGAAGCTTTGCTGGCAAATTCCAATCAGGATTGGGGTTACTTTGAGACTTTTATTAGTGAGAGTAAGATTGAGCTACTTGACCTCTGTTCCAAGAATGAACTGTCTGTTAACCTTTTCTCTGAAGAAGATGTGGAGAACTATATGTTCGATGATGATGAGTCAACCCTGGGCAGTGATGTCTGCTCCCTCAAAATTCGATATGAGTCCTTTCAGGACAATGTTCGAGACAAGACCACTCTTCTGATGCAGGAAGATGCCCAATTCAATTTTTTCCCCAGTGTCTTTACTACGTGTCCCAAGCGGGAATCTAAGAGTGGAATCCTCAAGCAGAGCAGTGATCTTTCTCAATTCAAGGTCCCTGATGTGAGCATCATCTGGGGAGAAGAAGATAAAAACTTGGACAAGAAGAAAGGCAAAGAGGAAGTACATGAAGACAAAAGCATAGAGACAAAAGATGAAAAAGATAATGGAGAAAAGCCTGCATTAAACAATAAACCATGTGGTGGTCTTGAGGTAGAGCAATTTAAGAACCTAAAGGCTGACCAACTTACTAATTCCCTGGAGACATCAGGGAATTTCAGTGATGACAGTTCCTTCATTGAGGTCTCATATGATGCCATGGGGGAGATCAAAGACTGTAGCCGCTATATGGCTCGGGACACTAATTCTGGAAGTTCATCCTCCCAGCAGAACTACGGACTGCGAGCCAAGAGAAAAGTCAGATACAGCGAAGATTATCTATATGATGTTGACTCGCTAGAGGGGGAAAAAGTAAATGAGAGAAAGGAATGGCCTCCAGGTGGTTCCAAAGAAGAAGATGATGATGAATGGTGTCCCAAAAAGAGAAGAAAAGTAACACGTAAAGAGCCCCCTGTTATTATTAAATATATCATTATCAATCGTTTTAAAGGTGAGAAGAACATGTTGGTGAAGCTAAGTAAAGTAGATGCCAGTGAAACAACAGTAAATCTGAGTGAGAATCAGCTCAGTAAGTATGCCAAGCTATCACCTTTGAAAGGATTCTGGCAAAAGAAGAAAAAGCAGAAAAACAGCAACACAGACTCAGTCAAAACACCCTTATGCCAAAAGCAAAGCTTTGAACCAGGTAGCTTTGAGGTGTCATTCCTGCCTCCTGCTCGTAAACGAAAATCTAAGCTTGGCAACAGACACAGGATTCAAAGAATCCAATCTGTGGAAACTTCAGCAAGTAGTAAGCAGGTTTCATTCTGCAGTGATCAGAAACAAGCTTGTAATCGCAAAGAAGACGGTGTAAAAGGGACACCAAAATCAGCACTTCTTACTGACCCAAGCTGTGCCAATGGATCACATTTACGTGGCCTCATAGTCTCCGACTCGGTTAAAGTCAAAGCTCAAGATACAGAGTTCAAGGGCCCAGAGAGGAAAGTGCTTAACAAAATTAAATTTAAAAGTGAAGCGCGCTTAAAATCCAAAAAAATCAAAGCTGGACAAGAAAACAAACCAGTTGTTCAAATGAGCCCTGTCTCAGAAGACACATCCTCCAAGGCTAATTTAAAAAATGAAGTCACTCCTGGAACCTCAAACAGTTCCCATATGTCTGAATTTCACGAGACAAAAGTTAAGAATTCCACTTTCCTACCAACCACCTGCTCTTCTGAAATGCCTCTATCATCTGCTAATGTTGCCACTAATATACCTGTTATTCCTGGAGGGTATCTGCAAACACTGTTAGATGCTTCTGACTTATCAAATAATACTAGTATCTCATACTTCACCAACCATTCTGCAGAGCAAAATGAAGGCAGCCTCACTCAAACGGAAAAAGCATTTGTACCTCTCCAGTCTGCTCAAGACTGTGTGCTGTCTTCATCTTCTGATTCTCAGCTGCAACAATCATCTCAGAACTTCAAAATGGAAGCAAGCAACTTTGGAAGTTTGTGGCCCGACAAGGATACATCTGGCTCCCAGGAATTCATGACTGAAGTCTCAAGGGAGATAGCCACAAACCAATCTAGTGAATTTGAAGCTTCCCAAGTTGTCTCCATGGAGAATAACCTCACAGCTATAACATACAGTCCTGTATGCCTCAATAGTGATGCCAGTGGTTGCAACAAGGTTCTGTATGCCTCTTTGCAGGACTCACATCTCCCACCTGAAGACTTGTATCAATTGTGTCACTTTAATAATGGAGAGATCTGCTTTCCGTTCCAGCAGGGCCCACTCAGTACAGATGATGATGGCCGGCTCTTTAGCTTTGATTCGATGACTTCCCTTACTGTTAGTTCAAGCAATTATTGTTCCTTAAGCTTGAAATCCTGTGAAAAGGATGGTGATGATGAAATCAATGATGACTTTCTGGCCCACTGCAGCCCCAAACTGGTGATCCAACAGAGCATTGATGAAATAGCACCACTAAAGGAGTCCACTGACCTCCTTGACATCTCCAACTTCACTCCTGACAAGTTCCGACACTCTTCTCTCTTAGAAATGTCTCCCCCAGACACCCCCAGTCTTTCTCCTCAGAGTACCAGATGTGAGAGTATCAAAACACTAGGAACAATGAAAGGATTCCAAGAAGGTGTCCCAGGATCGTTGAGCACCGTGGAAAAAATCAAGTGGGACTGTAATACCCTTTCACAACAGGCCCAAGCAGATGATGGCTTTACATTAAATAGCCATCAGTTTCAGTTCCATATGTTCAATGATGAGGACTCTGTTGGCCTGCTCCAAAAAAGCCCTTGCCTGTCAACATTTGATGAGCCAGCTGGGCAAATTAATACCAACAGCAAAGTGTCAAAATCAAGAAAGAAAACTTCACCTGGCAAGAGTGGGGCTGTAAGCCAAAGTTCTTCTCAGAAAAATTCCAGGAAAAAATCGCCAAAAGCAAGCAACAAAGGGGTTGAAAAGCCACCTAGCAAAACCTCCCGCCAGGTTCCTAAATCAACAAAGAAAGGGAAATATGTGGCTGCTGTCAATGGAGAGAAAATGCAAATTGGGATTGGCCATAGTGGGGGCCAACCCAATAGTACATCCTCCAATGCAAAGACACTGACTGAATGTATTCAGCATGGTGGTCCTGTTGCCTCTATGAAGATACCAAGTCAGAAGGGACTTTCTGGAGATTGGGCTTTGGGAAAGGAAAGCAGGCCAGGCTGGAATGACATGAGTGTAGTCACTAATACCAACAACCTTCTGGATGATGACCAACGAGAGTTTCAAGAGCCTTCCTATATCTTATCCAATATTGCCTCTGGTATGGCAGATGTGCAAAGATTCATGATGGCCTCCATGGAGCCCCTTTGGGAACCCATGGAGCACCAAGGGGAGTCCAACACATTCTACTCCCCTGATTCCAATAGCCTAAAATTAAAAACCCTCAAAATACTGGCTGGAACACCACAAGAATCTAAGAAAAAGGTTACTAATGGGTCTTCAGGAGCTACTAAGAATCACAGGTCAGTCAAAGCTGTGAGCAAAAGCAACGGGAAAGCGGCAATTGGTGAGCCTGGTCATGCAGATATGCCTGGTTCCAGTGAGGACTCTCGATCTGCCTTCTTTGATAAAAAGTACAGTAATGTGAACACTTTAGGCAATAATGGACCAACACACAAAAAATTATATCGTCACAAATCCAGCTCCAAGGGCCTGCGAGATGAGAAATATAAGGGAAAGCGCGTGGAACGAGAACAGGCCCACAAGGATGAGGCTGGAACAACTTCTTTTGAAAAACTGAGGGATTCCAACTACAATCTCCTAAAAGCAGAAACAGCATTTGGGGTTTTACCTGTGTTTGAAGAAGAGACTCACATTTTCCAGAAAGACATTTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDNQQ
DKVIAASANGDNNLINGVKNNDSEDQEVAMKSFVALEATTPIQPIPVIQK
ESPMFPRGLLPPPSKKPCMQSPPSPLALIEAPDHSANSASVNAISLTSGV
AKGLNTWSLPNECEKAPFAIMEPAGMSALNGDCLMQPSRTCLGCFMESKE
AVDPEPGISLKVSDLNRDYETCAVSDIGIQCINAGENIKYGEQLLSDQLL
GFPLHKSRAGDRRESEKPDIDLEDPTQKSYYEALLLDKCNTEEALLANSN
QDWGYFETFISESKIELLDLCSKNELSVNLFSEEDVENYMFDDDESTLGS
DVCSLKIRYESFQDNVRDKTTLLMQEDAQFNFFPSVFTTCPKRESKSGIL
KQSSDLSQFKVPDVSIIWGEEDKNLDKKKGKEEVHEDKSIETKDEKDNGE
KPALNNKPCGGLEVEQFKNLKADQLTNSLETSGNFSDDSSFIEVSYDAMG
EIKDCSRYMARDTNSGSSSSQQNYGLRAKRKVRYSEDYLYDVDSLEGEKV
NERKEWPPGGSKEEDDDEWCPKKRRKVTRKEPPVIIKYIIINRFKGEKNM
LVKLSKVDASETTVNLSENQLSKYAKLSPLKGFWQKKKKQKNSNTDSVKT
PLCQKQSFEPGSFEVSFLPPARKRKSKLGNRHRIQRIQSVETSASSKQVS
FCSDQKQACNRKEDGVKGTPKSALLTDPSCANGSHLRGLIVSDSVKVKAQ
DTEFKGPERKVLNKIKFKSEARLKSKKIKAGQENKPVVQMSPVSEDTSSK
ANLKNEVTPGTSNSSHMSEFHETKVKNSTFLPTTCSSEMPLSSANVATNI
PVIPGGYLQTLLDASDLSNNTSISYFTNHSAEQNEGSLTQTEKAFVPLQS
AQDCVLSSSSDSQLQQSSQNFKMEASNFGSLWPDKDTSGSQEFMTEVSRE
IATNQSSEFEASQVVSMENNLTAITYSPVCLNSDASGCNKVLYASLQDSH
LPPEDLYQLCHFNNGEICFPFQQGPLSTDDDGRLFSFDSMTSLTVSSSNY
CSLSLKSCEKDGDDEINDDFLAHCSPKLVIQQSIDEIAPLKESTDLLDIS
NFTPDKFRHSSLLEMSPPDTPSLSPQSTRCESIKTLGTMKGFQEGVPGSL
STVEKIKWDCNTLSQQAQADDGFTLNSHQFQFHMFNDEDSVGLLQKSPCL
STFDEPAGQINTNSKVSKSRKKTSPGKSGAVSQSSSQKNSRKKSPKASNK
GVEKPPSKTSRQVPKSTKKGKYVAAVNGEKMQIGIGHSGGQPNSTSSNAK
TLTECIQHGGPVASMKIPSQKGLSGDWALGKESRPGWNDMSVVTNTNNLL
DDDQREFQEPSYILSNIASGMADVQRFMMASMEPLWEPMEHQGESNTFYS
PDSNSLKLKTLKILAGTPQESKKKVTNGSSGATKNHRSVKAVSKSNGKAA
IGEPGHADMPGSSEDSRSAFFDKKYSNVNTLGNNGPTHKKLYRHKSSSKG
LRDEKYKGKRVEREQAHKDEAGTTSFEKLRDSNYNLLKAETAFGVLPVFE
EETHIFQKDI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45278
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee