cDNA Clone UBQLN2 (mouse)

• Plasmid: pET15 6HIS SUMO UBQLN2 (mouse)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS-SUMO1-UBQLN2
• Genbank: NM_018798.2
• Tag: 6His
• Tag: SUMO
• Species: Mouse

Sequence of Insert

GGATCCATGGCTGAGAACGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCGCGGCCCTGCGGCGGCCCCAGGCGCGGCCAGCCCGCCGGCCGAGCCCAAAATCATCAAAGTCACTGTGAAGACCCCCAAAGAGAAGGAGGAGTTCGCGGTGCCCGAGAACAGCACCGTGCAGCAGTTCAAGGAAGCGATTTCCAAACGCTTCAAATCGCAGACCGATCAGCTCGTGCTGATTTTCGCCGGAAAGATCCTGAAAGATCAAGATACCTTGATGCAGCATGGCATCCATGATGGACTGACTGTTCACCTGGTTATCAAAAGCCAGAACCGTCCGCAGGGCCAGGCCACCACGCAGCCCAGCACGACTGCGGGAACGAGCACGACGACCACGACCACCACCACGGCGGCGGCGCCGGCGGCGACGACATCATCGGCTCCCAGGAGTAGCTCCACACCTACCACCACGAATAGCAGCTCCTTTGGGCTGGGAAGCCTTTCCAGCCTTAGTAACCTGGGCTTGAACTCGCCCAACTTCACCGAGCTTCAGAACCAGATGCAGCAGCAGCTCCTGGCCAGCCCTGAGATGATGATCCAGATCATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCTGATCTGATGAGGCAGCTCATCATGGCCAATCCACAGATGCAACAATTGATCCAGAGAAACCCAGAAATCAGCCACCTACTGAACAACCCAGATATAATGAGGCAGACGCTGGAAATCGCCAGGAATCCTGCCATGATGCAAGAGATGATGCGAAATCAAGACCTGGCTCTCAGTAATCTCGAAAGCATCCCAGGTGGCTACAATGCTCTGCGGCGCATGTACACTGACATTCAAGAACCTATGCTGAATGCCGCACAGGAGCAGTTTGGGGGCAACCCGTTTGCCACGGTGGGGAGCAGTTCCACCTCAGGGGAAGGTACTCAGCCCTCCCGCACGGAGAATCGTGATCCGCTGCCCAATCCTTGGGCACCACCGCCAACAACCCAGACGGCTGCGACCACCACCACCACCACGACCACAAGCAGTGGCAGTGGCTCTGGCAGCAGCTCCAGCAGCACCACCGCCGGGAACACCATGGCTGCAGCTAATTACGTGGCTAGCATCTTCAGCACCCCAGGAATGCAGAGCCTGCTGCAGCAGATAACTGAGAATCCCCAGCTGATTCAGAATATGCTGTCTGCGCCCTACATGCGGAGCATGATGCAGTCGCTGAGCCAGAATCCAGATATGGCGGCCCAGATGATGCTGAGTAGCCCGCTGTTCACTTCGAATCCTCAGCTGCAGGAGCAGATGCGTCCACAGCTCCCGAATTTCCTGCAGCAGATGCAGAATCCAGAAACCATCGCGGCCATGTCAAACCCGAGAGCGATGCAAGCGCTAATGCAGATCCAGCAGGGGCTACAGACTCTAGCCACTGAAGCACCTGGCCTCATTCCCAGCTTCGCTCCAGGTGTGGGGATGGGAGTGCTGGGAACCGCCATAACCCCTGTGGGCCCAGTCACTCCCATAGGGCCCATCGGCCCTATAGTTCCTTTCACCCCCATAGGCCCCATCGGGCCTATAGGACCCACTGGCCCTGCAAGCTCCCCCGGCTCCACCGGCACTGGCATCCCGCCTGCAACCACTGTGTCCAGCTCTGCACCTACTGAAACCATAAGTCCAACCTCGGAATCTGGACCCAACCAGCAGTTCATTCAACAAATGGTGCAGGCTCTCACTGGAGGAAGCCCTCCACAGCCGCCGAATCCTGAAGTGAGATTTCAGCAGCAACTGGAACAGCTCAACGCCATGGGGTTCTTAAACCGCGAAGCAAACTTGCAGGCCCTAATAGCAACAGGAGGCGACATCAACGCGGCCATTGAGAGGCTGCTGGGCTCTCAGCCATCCTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSMAENGESSGPPRPSRGPAAAPGAASPPAEPKIIKVTVKTPKEKEEFAVPENSTVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLMQHGIHDGLTVHLVIKSQNRPQGQATTQPSTTAGTSTTTTTTTTAAAPAATTSSAPRSSSTPTTTNSSSFGLGSLSSLSNLGLNSPNFTELQNQMQQQLLASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFATVGSSSTSGEGTQPSRTENRDPLPNPWAPPPTTQTAATTTTTTTTSSGSGSGSSSSSTTAGNTMAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDMAAQMMLSSPLFTSNPQLQEQMRPQLPNFLQQMQNPETIAAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFAPGVGMGVLGTAITPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPASSPGSTGTGIPPATTVSSSAPTETISPTSESGPNQQFIQQMVQALTGGSPPQPPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22795
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs linker after senp cleavage deleted, Has silent mutation g681a; Parental Plasmid DU32993