cDNA Clone UBQLN3 (mouse)

• Plasmid: pET15 6HIS SUMO UBQLN3 (mouse)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS SUMO1-UBQLN3
• Genbank: NM_198623.2
• Tag: 6His
• Tag: SUMO
• Species: Mouse

Sequence of Insert

GTCGACATGGCCAAAAGTGGAGAGGCTCTACCACAGGGCAGCCTAGCGCCAGCCCAGGATTCCCAGCTCATCCGGGTGACAGTGAAGACACCCAAGGACAAGGAGGATTTCTCAGTTGTAGACACATGCACCATCCGGCAGCTAAAGGAAAAAATATCACACCGCTTTAAGGCACATCCAAATCAGCTGGTCCTGATCTTTGCTGGAAAAATCCTCAAGGACCCTGACTCATTGGCACAGTGTGGAGTGCGAGATGGCCTCACAGTCCACCTGGTCATCAAGATGCAGCGTCGGACCATAGGCACAGAGTGCCCATCACCACCAGTCTCTATTCCAGGCCCAAATCCTGGAGAAATCCCTCAGTCAAGTTCTGTTTACTCTGTGGATGGGTCTCCTTCTTTCAGCTTAGGGGTCCTCACAGGCCTCAGTGGGTTAGGTCTGACCTCAGGAAGCTTCTCTGACCAGCCAGGCTCACTAATGTGGCAGCACATCTCAGTGCCTGAACTTGTGGCTCAGCTCGTTGATGACCCC-TTTATCCAGGGTCT-GCTGTCCAACACGGGCCTAGTGCGCCAGTTGGTTCTTGACAATCCTCACATGCAGCACCTGATCCAGCAGAACCCTGAGATTGGTCATATTCTTAACAACCCTGAGATCATGCGGCAGACAATGGAGTTTCTTCGTAACCCTTCCATGATGCAGGAGATGATGCGTAGCCAGGATCGTGCACTCAGTAACCTGGAGAGCATCCCTGGTGGCTACAATGTGCTCCGTACTATGTACACAGATATTATGGATCCCATGCTTAATGCAGTACAGGAACAATTTGGTGGCAATCCTTTTGTCACTGCTACTACTGCTAGTACCACCACCACCAGCAGCCAACCATCAAGGACAGAGAATTGTGACCCTCTGCCCAACCCCTGGACTTCCACATATGGGGTCTCAGGTGGAAGACAAGGAAGAGGTGGCAGACAGTCTGGGGACCAGGATGCATCTGAAAATAGAAATAGGCTTCCAAGCTTTCTGGGTAACATAGGACTCTTTGATTATCTTCAACAGTTACATGAGACTTCTCAGTCCCTGGAATCTTATCTGCAGGGTACTGTGCCAACTTCAAATCCAAGCCAGGAATCACCACTTTCAGGAAACAGAGTTCCTCCAACTTTACCATCATCCCCTAAATCTGGGTCAGGCCAATCTCTGCCCAAAGAGTCAGTAGCAATCAAGGGAAAGTCATCATGCCCAGCTTTCTTGAGACACTCAACTGAGAACAGTACTGGACAAGGTGGAAGCCTACATGATGCAGGGAAGGGCTCTACTGGCCCCAGTACTAGCTTGCCCAATCTTACCTCTCAGATAGGAGATTCTGCCAACAGATCCTCGTTTGTTTCTACACCATCTTCCCTTATGTCAGCTACTCCTGGAGTCCCAGAATCTCCCTGGCTTCCTCCAACAGGTTACTCAAGATCTCTGAGATCAGCGGGCACAAATCAAGTCCCTCGAATCCAGAATGAGATACATCAACAGCTACCACTACTGTTGCATCTTCAGACAGCCATGGCAAATCCCCGTGTCATGCAAGCCTTGCTGCAGATTGAGCAGGGTCTACAGATTCTGGCTACTGAAGCACCCCGCCTCCTGCTCTGGTTCATGCCCTGCCTAACAGGGTTGAATGGAGTAACAGGAGGCACAGAAGCTAGAGAAGGTGTTGTTATGTCTGAGGATCCCCGACCAACCCCAACTCCTCAGATTTCTCTAGCACAAGGTTCTACAGAGCTAGGTATCCATTCCTCCCCCTTCCTCCAGGTGTTACAAGCCTTAGCTAGTACCAATCCCCAGCAGCTACAACTGGAGGCTCACTTCCGGGTACAACTGGAGCAACTGCGGGCTATGGGCTTTCTGAATCTTGAAGCCAATCTCCAGGCCCTCATTGCTACAGAGGGTGATGTAGATGCTGCTGTGGAGAAATTGAGGAAGTCTTAGGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGVDMAKSGEALPQGSLAPAQDSQLIRVTVKTPKDKEDFSVVDTCTIRQLKEKISHRFKAHPNQLVLIFAGKILKDPDSLAQCGVRDGLTVHLVIKMQRRTIGTECPSPPVSIPGPNPGEIPQSSSVYSVDGSPSFSLGVLTGLSGLGLTSGSFSDQPGSLMWQHISVPELVAQLVDDPFIQGLLSNTGLVRQLVLDNPHMQHLIQQNPEIGHILNNPEIMRQTMEFLRNPSMMQEMMRSQDRALSNLESIPGGYNVLRTMYTDIMDPMLNAVQEQFGGNPFVTATTASTTTTSSQPSRTENCDPLPNPWTSTYGVSGGRQGRGGRQSGDQDASENRNRLPSFLGNIGLFDYLQQLHETSQSLESYLQGTVPTSNPSQESPLSGNRVPPTLPSSPKSGSGQSLPKESVAIKGKSSCPAFLRHSTENSTGQGGSLHDAGKGSTGPSTSLPNLTSQIGDSANRSSFVSTPSSLMSATPGVPESPWLPPTGYSRSLRSAGTNQVPRIQNEIHQQLPLLLHLQTAMANPRVMQALLQIEQGLQILATEAPRLLLWFMPCLTGLNGVTGGTEAREGVVMSEDPRPTPTPQISLAQGSTELGIHSSPFLQVLQALASTNPQQLQLEAHFRVQLEQLRAMGFLNLEANLQALIATEGDVDAAVEKLRKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22807
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs linker after SENP cleavage deleted; Parental Plasmid DU32993