cDNA Clone UBQLN4 (mouse)

• Plasmid: pET15 6HIS SUMO UBQLN4 (mouse)
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6HIS-SUMO-UBQLN4
• Genbank: NM_033526.2
• Tag: 6His
• Tag: SUMO
• Species: Mouse

Sequence of Insert

GGATCCATGGCGGAGCCGAGTGGGGCTGAGACGAGGCCCCAGATTCGGGTCACCGTCAAGACCCCCAAGGACAAGGAGGAGATCGTGATCTGCGATCAAGCCTCGGTCAAGGAGTTCAAGGAGGAGATCTCCCGGAGGTTTAAGGCTCAGCAGGATCAGCTGGTCCTGATCTTCGCAGGCAAGATCCTCAAGGATGGGGACACCCTGAGCCAGCACGGCATCAAGGATGGGCTCACAGTCCACCTAGTCATCAAAACCCCTCAGAAGGCTCAAGATCCAGTGACTGCTGCTGCTTCTCCTCCATCCACTCCTGACTCTGCCTCTGCACCCTCCACCACGCCTGCCTCACCTGCCGCCGCCCCTGTCCAGCCCTGCAGCTCTGGCAATACAACTTCAGATGCTGGCAGTGGAGGGGGGCCCTCTCCAGTGGCTGCAGAGGGGCCCTCAAGTGCTACTGCATCGATCCTCTCTGGCTTCGGAGGCATCCTGGGACTGGGCAGCCTGGGCCTGGGCTCCGCCAACTTTATGGAGCTGCAGCAACAGATGCAGAGACAGCTCATGTCCAATCCTGAGATGCTGTCGCAGATCATGGAGAACCCCTTGGTCCAGGATATGATGTCAAACCCTGATCTGATGCGCCACATGATCATGGCTAATCCCCAGATGCAGCAGCTGATGGAGAGGAACCCCGAGATCAGCCACATGCTCAACAACCCAGAGCTCATGAGGCAGACAATGGAGCTTGCTCGTAACCCAGCCATGATGCAAGAAATGATGCGGAACCAGGACCGGGCCCTCAGCAACCTGGAAAGTGTCCCGGGAGGGTATAATGCCCTCCGCCGCATGTACACGGACATCCAGGAGCCCATGTTCACTGCTGCTCGGGAACAGTTTGGCAACAATCCCTTCTCTTCCCTGGCCGGGAACTCCGACAACTCATCCTCCCAACCTCTTCGGACTGAGAATCGAGAGCCCCTCCCTAACCCCTGGAGCCCCTCGCCCCCCACCTCCCAGGCCCCCGGGTCCGGTGGGGAGGGCACCGGAGGATCGGGGACCAGCCAGGTGCACCCGACAGTCTCGAACCCCTTTGGGATCAATGCGGCTAGCCTGGGGTCAGGGATGTTCAACAGCCCAGAAATGCAGGCGCTCCTCCAGCAGATTTCTGAAAACCCCCAGCTGATGCAGAATGTCATCTCAGCCCCTTACATGCGCACCATGATGCAGACGCTTGCCCAGAACCCTGACTTTGCTGCTCAGATGATGGTGAATGTGCCGCTGTTCGCAGGGAATCCACAGCTTCAGGAGCAGCTCCGCCTGCAGCTCCCCGTGTTTCTGCAGCAGATGCAAAACCCAGAGTCTCTCTCCATCCTCACCAATCCCCGGGCCATGCAGGCCCTGCTGCAGATCCAGCAGGGACTACAGACCCTCCAGACTGAAGCCCCTGGGCTGGTACCCAGCCTTGGCTCCTTTGGGACACCCCGGACCTCGGTACCTCTGGCAGGCAGCAACTCTGGATCTTCGGCAGAGGCCCCCACTTCTTCCCCGGGCGTGCCAGCCACATCTCCTCCCAGTGCGGGTTCCAATGCCCAGCAACAACTCATGCAGCAGATGATCCAACTTTTGTCCGGAAGTGGGAACTCACAGGTGCCGATGCCAGAAGTGAGATTTCAACAGCAGCTGGAACAACTCAACTCCATGGGCTTTATTAACCGTGAGGCCAACCTACAGGCCCTGATCGCCACAGGAGGGGACATCAATGCCGCTATTGAGAGACTCCTGGGCTCCCAGCTCTCCTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSMAEPSGAETRPQIRVTVKTPKDKEEIVICDQASVKEFKEEISRRFKAQQDQLVLIFAGKILKDGDTLSQHGIKDGLTVHLVIKTPQKAQDPVTAAASPPSTPDSASAPSTTPASPAAAPVQPCSSGNTTSDAGSGGGPSPVAAEGPSSATASILSGFGGILGLGSLGLGSANFMELQQQMQRQLMSNPEMLSQIMENPLVQDMMSNPDLMRHMIMANPQMQQLMERNPEISHMLNNPELMRQTMELARNPAMMQEMMRNQDRALSNLESVPGGYNALRRMYTDIQEPMFTAAREQFGNNPFSSLAGNSDNSSSQPLRTENREPLPNPWSPSPPTSQAPGSGGEGTGGSGTSQVHPTVSNPFGINAASLGSGMFNSPEMQALLQQISENPQLMQNVISAPYMRTMMQTLAQNPDFAAQMMVNVPLFAGNPQLQEQLRLQLPVFLQQMQNPESLSILTNPRAMQALLQIQQGLQTLQTEAPGLVPSLGSFGTPRTSVPLAGSNSGSSAEAPTSSPGVPATSPPSAGSNAQQQLMQQMIQLLSGSGNSQVPMPEVRFQQQLEQLNSMGFINREANLQALIATGGDINAAIERLLGSQLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22809
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs linker after SENP cleavage deleted; Parental PLasmid DU32993